SEDGWICK AND WINSLOW. 
BACILLUS OF TYPHOin FKTLU. 
501 
Race D. 
Series L 
I 
Number 
of Tube. 
101 
102 
103 
104 
10 J 
106 
107 
108 
109 
110 
131 
132 
133 
134 
135 
136 
137 
138 
139 
140 
Average Number Bacteria per c.c 
Unfrozen Water. 
78435 
76230 
1765 
2730 
275 
7735 
1120 
11690 
6895 
3500 
29190 
20160 
6055 
5710 
3885 
Thawed Ice. 
147 
451 
23 
47 
15 
26 
49 
134 
235 
175 
77 
371 
192 
388 
144 
Reduction 
per cent. 
99.8 
99.4 
98.7 
98.3 
94.5 
99.7 
95.6 
98.9 
96.6 
95.0 
99.7 
98.2 
96.8 
93.2 
96.3 
Average 97,4 
Tubes 101-110, 
frozen in 3 hours. 
Tubes 131-140, 
frozeu in 3 hours. 
frozen May 5, 1899;' tliawed and 
Minimal temperature, (—13° C). 
frozen May 13,1899; tliawed and 
Minimal temperature, (-19° C). 
re- 
re- 
Nntnber 
of Tube. 
Ill 
112 
113 
114 
115 
no 
117 
IIH 
119 
120 
121 
122 
123 
124 
125 
126 
127 
128 
129 
130 
Kace 1). 
Si 1!I 
Averife KuuiUT IW'Uirik par c.c. 
Unfrozen W:<iir. 
34020 
r.'.io'ju 
3jn25 
1 54 5 
4270 
2095 
GGS5 
5250 
13C85 
203 1 75 
4 0005 
56070 
170100 
925 
Accra ge . 
Tlaiu J lue. 
71 
1 52 
7 
274 
9 
20 
1 
5 
OS 
21(> 
3 
1 
o 
r 
207 
1 
11. 
J. 
90.7 
90.4 
<x>3 
0R.7 
98.5 
!W94- 
998 
99.9 
rin 5 
Tubes 111-120, frozen May O.lS'ifl; tliiiwH and rcjW-'-i 
twice in next <i honrs. Minini.il tt'ini*crature, ( U°C.). 
Tubes 121-130. frozen May 10,1891»; thawed and wfmtm 
twice in next O hours. Minimal temperature, ( -10' C ). 
C. EXPEKIMENTS ON THE EFFECT OF TEMPERATURES SLIGHTLY ABOVE THE 
FREEZING-POINT UPON TYPHOID BACILLI IN 
WAT I 
In these experiments sterilized test tubes were Inoculated with pure ci 
Afterward they were treated in one of three wa} 
preceding work 
e 
itlicr 
placed in 
from 8-1 
an 
ubator at the room temperati 
J 
20' C, or in an ice-chest ranging 
V, or cooled in the freezer 
effected by filling the outer chamber 
point just above freezing 
T 
was 
In the three sets of tubes treated by the last method at 1°, the duration 
sure 
d the reduction 
as follow 
Race A In two hours was reduced 47-8 per 
Race B in one and one-half hours 
red 
32.9 percent; Race C 
duced 80.1 per cent. 
The 
reductions for the same races actually frozen 
for the nearest correspond 
periods, were 73.6 per cent, 41.4 per cent, and 
99 
cent, respectively 
tion in the chilled 
Each race maintains its relative positi 
IS very 
ly as g 
the 
ily what the temperature difference might be expected to prod 
Therediic 
d the dilicrence ii 
I. Evidently tbert 
