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T 
I 
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SEDGWICK AND WINSLOW. — BACILLUS OF TYninlD FEVEIL m.') 
TinioiD Bacilli in Eaiitu. Avkraot^ NiMnr.R vvm GraM. 
After Inoculation. Aftiir 1 day. 3 day*. 7 <1iir». U <Us 
Series 10° 18077G 4n;;5 705 1'5 *♦ 
It 0° 484G855 95017 13'J5 5L'5 f.ss 
III O'^ 7778595 324588 4656 1304 lit." 
IV 20° 4673683 25G5 450 05 iC 
Two more series of experiments with earth were carried out to thruw light uii 
the part phiyed by dryness in tlie reduction manifest in the first experiments. In 
these latter researches the sets of fifty tubes were inoculated ju«t ;ls before, and ten 
of them were planted at once.* The remaining forty were divided into two pnrtlun'=' 
The gram of earth in each of twenty of the tubes was moistened by tlic nddifion of 
about one-third cubic centimeter of sterilized tap water; while the earth in the <>Hkt 
twenty tubes was left in its comparatively dry condition. The tubes wrro all kf*pt at 
the room temperature. Thus a comparison may be drawn as to the vinbility of the 
germ in damp and in dry earth. The results were as follows: 
Typhoid Bacilli in Dry and Damp Eautii. Avera^.l Nimi.kk rtu T.kam. 
After Inoculation. 
AfU'r5<Uy. 3 day*. 7 <U>». 14<l«y». 
Series V 939115 
fDry 2070 50 2 h 
I Damp 225 7010 1205 8 
fDiT 566 71 12 4 
^I ^'''''' { Damp IGOOllO - - 205S7 
Conclusions. 1. The typhoid bacilli in dry earth behave just a.s in wal.r mid 
in ice. They die out, rapidly at first, and their numbers arc progressively reduced 
ed. A fraction of one per cent persists fo 
ne treatment is prolonged. A traction oi one pci vc"«> i— 
2. Cold alone does not materially affect the reduction of typhoid germs in dr) 
earth. 
3. In moist earth, although the main phenomena are 
dc«t ruction 
bactci-ia is .nucli less rapid. With the liberal fooJ supply introJuced ». 
bouillon in these experiments, they appear sometimes to hold their own c.ituely 
