I70 BOTANICAL GAZETTE. [J^ty? 



Returning for a moment to the cultures of G. clavariae- 

 forme, it is of interest to note that one of the plants infected 

 in 1887, on which the Rcestelia had developed with great 

 luxuriance, was left out of doors over night during a rain, in 

 order to observe the effect thus produced upon its general 

 habit. Before this exposure the peridia had remained un- 

 broken, as is usually the case with cultures carried on indoors ; 



g*>t 



induce the 



R 



This circumstance removes any doubts in connection with 

 my determination of this species which may have rested 

 on the failure of my previous culture to assume a lacerate 

 habit. It should be mentioned here that in the Bulletin of 

 the Iowa Agricultural College for 1887, Prof. Halsted de- 

 scribes a culture of G. clavarieeforme made by him out of 

 doors upon Amelanchier with material stmt from the east, 

 which resulted in the production of a Rcestelia which he re- 

 ferred to R. botryapites, thus throwing doubt upon my results 

 both with G. biseptatum and G. clavaria±forme. Prof. Far- 

 low has kindly allowed me to state in this connection that 



Rcestel 



Halsted as re- 



sulting from this culture is certainly not R. botryapites. That 

 my first result was correct is sufficientlv shown by my subse- 

 quent cultures, as well as by the conclusions olf European 

 experimenters. It is, moreover, quite improbable that a Gym- 

 nosporangium, also well known in Europe, should produce a 

 Rcestelia which occurs only in America. 



During the summer of "1887 I had some opportunity of 

 observing the Rcestelias occurring in the mountains of North 

 Carolina, concerning which a note may be of interest. At 

 Cullowhee, in the southwest portion of the state, and else- 

 where in the same region, cedars (J. Virginiana) were not 

 indigenous, but had been introduced in small numbers, and 

 were inmost cases loaded with "cedar apples" to a degree 

 seldom if ever met with in the North, and in one case, near 

 the town of Sylva, two large treesjseemed to have been literally 

 killed by them, while in several other cases their detrimental 

 effect was apparent. The season of their maturity was 

 passed at the time (June), but the species were readily recog- 

 nized as G. macropus and G. globosum, both attaining a re- 

 markable size. I noticed, however, no conspicuous distor- 

 tions referable to our " birds'-nest " species. Of Rq stelias 

 I tound three forms. One on Crataegus Crusffalli, collected 



June 13, I have been unable 



