2I 4 botanical gazette. [September 



g7. V 



become separated from the spore, and after a time send out 

 tubes, like those which come from the sporidia when they 

 germinate. This method is seldom found among the spores 

 on the outside, but occurs chiefly in those in the inner part 



of the spore-mass. 



In the forms of germination already considered, it will be 

 seen from the accompanying figures and descriptions that 

 the promycelia arose from the region of the septum in all 

 cases, no matter whether the spore was obtuse or acute, or, 

 in other words, whether it was what Kienitz-Gerloff calls a 

 teleutospore or a uredospore. We come now to other forms 

 of germination, where the germinal tube arises from the apex 

 of the spore instead of from the region of the septum. This 

 method of germination was found beyond a doubt in both the 

 acute and obtuse forms, though more frequently in the latter. 

 In fig. 8 a spore which is clearly of the acute form is repre- 

 sented germinating by the end, while in fig. 9 one of the ob- 

 tuse kind is shown behaving in a similar manner. This form 

 of germination of the obtuse spores was apparently not seen by 

 Kienitz-Gerloff. In some cases where germinal tubes were 

 produced from the end of the spore promycelia were also 

 found arising from the region of the septum. 



The terminal promycelia grew in all respects like the 

 others, and, with few exceptions, they all bore sporidia. 

 No cases were seen where the germinal tubes grew out to 

 any very great length even when the spores had been kept 

 in an excess of water. In one case, where the spores were 

 only kept damp, a terminal promycelium was noticed, which 

 broke up in the same way as those arising from the septum 

 under similar conditions (fig. 10). On two occasions ger- 

 minal tubes were seen to arise from the fixed end of the 

 spore, very near the point of attachment of the pedicel, 

 but, except for their point of origin, the promycelia were 



normal. 



If we now compare the results of my observations with 



those of Kienitz-Gerloflf, it will be seen that the latter are not 

 sufficient to warrant the conclusions which he drew irorn 

 them. From both his observations and my own it is plain 

 that the obtuse spores borne on the outside of the spoie- 

 masses are teleutospores, since, on germinating, they pro- 

 duce the characteristic promycelia. Apparently Kienitz- 

 Gerloff is of the opinion that the promycelia are always pro- 

 duced at the septum. I have shown, however, that, although 



