squares. The leaves are simple, and for this reason, as before 
mentioned, it was necessary to observe the greatest exactitude 
The figure of 1.27 gr. for the quantity of dry matter, produced 
in each hour per square Meter, was got by subtracting the 
morning weight from the 12 o’clock weight and dividing the 
rest by the number of hours. : 
It cannot fail to be observed that the morning and evening 
weights are almost equal. From this the inference would be 
that the transmission of amylum during the night must be d 
little account. As a matter of fact it appeared from several 
observations that the loss both as compared with the figures | 
found by Sachs, and also considered by itself is slight. : 
Sachs found for Helianthus annuus 0.964 or. 
» Cucurbita Pepo 0.828 gr. i. 
According to the above it is only 0.23 for Connarus faleatus. 
Besides, it has already been pointed out that in the early 
morning most of the leaves contain a pretty large quantity 
of amylum judging from the results of the iodine experiment. 
The following experiment still more clearly shows the loss of 
amylum during the night: on May 12 at 11.55 a. m. a few 
leaves of Delima sarmentosa were subjected to the iodine § 
periment; their colour became dark blue; at the same time 
stout leafy branch of the same specimen was put into a dé 
cupboard and left there till the next morning at 7.10. Wi 
tested with iodine, the leaves were found to have lost hardly 
any colour. Still they had been in the dark for upwards of 
hours at a temperature of 21° C. at the very least. 
In the same way I cut off a few leaves of Delima at 
p- m. on May 12; these got a light blue colour. Others cut 
at the same hour but placed in the dark cupboard, were 
any lighter in colour. Consequently the leaves of Delima pla¢ 
in the dark lose in 19 hours much less than in 4 hours W™ 
exposed to the sunlight; can this be owing to the higher 
perature only? It is hard to believe it ge 
J) In these experiments on purpose stout branches were taken to facilitate 
es of amylum as much as possible; still I am not prepared to say ' 
doing so my object was fully attained. 
