23,5 Leach et ah: Hookworm Infestation 461 



was administered in twice its volume of cold water at about 

 1.30 o'clock in the afternoon, the men being immediately marched 

 into the cell house and made to lie down until the bowels began 

 to move. One or more of the authors remained to watch the 

 men and to take and record the pulse and blood pressure of 

 each man one hour after treatment. Such of the men as cared 

 to do so were allowed to eat their evening meal at about 5 

 o'clock. Stools were collected for three days and carefully 

 screened to recover the worms, after which the men were re- 

 leased from quarantine. 



The stools were washed through a screen of sixty meshes 

 to the inch. This probably was finer than necessary, but it 

 was the only screen available in Manila. The worms were pre- 

 served in 70 per cent alcohol and later cleared in 10 per cent 

 glycerine-alcohol. 



Reexaminations of the blood and the urine were made twenty- 

 four hours after treatment, and in a considerable number of 

 the cases the blood was examined a third time. Eventually, 

 the stool of each man was examined microscopically to deter- 

 mine if he had been rid of his worms. 



Several men in the series were later deported and some others 

 were transferred to a penal colony, so it was not possible to 

 carry out a series of test treatments. 



HELMINTHAL FINDINGS BEFORE TREATMENT 



While it was desired principally to study the effects of carbon 

 tetrachloride on hookworm infestation, it was thought wise to 

 include a few subjects in which that parasite was not present. 

 Of the men selected for experiment eighty-nine were found, on 

 microscopical study, to harbor hookworms. The remaining 

 eleven were infested with Ascaris, Trichuris, or both. Infesta- 

 tions with Ascaris to the number of fifty-three, and with Tri- 

 churis to the number of eighty-four, were found in the group. 

 These infestations were distributed as shown in Table 2. 

 Table 2— Distribution of helminthal infestations. 



Trichuris only 6 



Ascaris and Trichuris 4 



Hookworm only 9 



Hookworm and Ascaris 6 



Hookworm and Trichuris 31 



Hookworm, Ascaris, and Trichuris 43 



Oxyuris 1 



