Schbbl: Chaulmoogra and Allied Preparations 535 



ehaulmoogra oil toward acid-i 



3. The chaulmoogra therapy of experimental tuberculosis in laboratory 



animals with particular reference to resulting immunity. 



4. The antiseptic effect upon acid-fast bacilli in vivo of such organic 



compounds as have been found to have a high value in vitro. 



Results are given in this paper and in papers being written of 

 my own experiments which were continued along this line as 

 a part of the research program of the Philippine Leprosy Re- 

 search Board. 



The technic employed in the following experiments was very 

 simple. Measured amounts of the oils were added to test tubes 

 containing 10 cubic centimeters of 5 per cent glycerine agar and 

 kept in boiling water for thirty minutes. They were then 

 vigorously shaken and cooled quickly in a slanted position. 

 When solidified the tubes were kept in an upright position 

 overnight to drain off the water of condensation. After plant- 

 ing the cotton plug was sealed with paraffin. Incubation of 

 planted tubes followed, readings being made in two and four 

 weeks. With each set control cultures were made, one on plain 

 glycerine agar and one on glycerine agar containing olive oil. 

 In the tests of sodium salts of fatty acids a solution of green 

 soap was used as a control. The dilutions of oils were made 

 in olive oil, those of soaps in distilled water, and the total 

 amount of dilution added to one tube was usually 0.1 cubic 

 centimeter, never exceeding 0.5 cubic centimeter to a test tube. 



The acid-fast culture used in these experiments was an old 

 laboratory strain of Bacillus tuberculosis, human type, originally 

 obtained from the Kitasato Institute for Infectious Diseases in 

 Tokio. The culture grew well on slightly acid beef infusion 

 agar to which 5 per cent glycerine had been added, producing 

 a rather moist, well emulsifiable confluent growth, which was 

 noticeable in from eight to ten days after planting. In inocu- 

 lation of the medication and the control agar tubes a small 

 loopful of young growth was placed on the surface of the slant 

 to be inoculated, and distributed well over the entire surface 



