538 The Philippine Journal of Science 1923 



In order to make Table 3 intelligible, the method of prepa- 

 ration of the various soaps used in these experiments is taken 

 from a paper by G. A. Perkins, 5 whose preparations I used. 



Sodium gynocardate A. This is the derivative of chaul- 

 moogra oil used for subcutaneous and intravenous injection since 

 1917 by Sir Leonard Rogers. 



Sodium gynocardate S. This preparation was made in the 

 same manner as sodium gynocardate A, except that the total 

 fatty acids instead of the crystallized acids were employed. 



Sodium gynocardate D. Differs from sodium gynocardate S 

 only in that the fatty acids have been purified by distillation in 



The sodium morrhuate was prepared by Rogers's method. 

 This consists in making the sodium soap of cod-liver oil and 

 extracting with ether to remove irritating substances. 



The results of experiments tabulated in Table 3 confirm the 

 findings of Walker and Sweeney in their Table VII. It shows 

 that sodium gynocardate S, a soap made of the total fatty acids 

 of chaulmoogra oil, gives the highest antiseptic value; sodium 

 gynocardate D (distillate) is next strongest, and sodium gyno- 

 cardate A (Rogers) is not so strong as the other two soaps 

 mentioned. Due probably to different technic, the differences 

 in the effect of the various preparations are not so pronounced 

 as in the experiments of Walker and Sweeney, but they are 

 distinct. 



The soaps made from isolated acids of chaulmoogra oil, 5 that 

 is, sodium chaulmoograte and sodium hydnocarpate, show lower 

 value than do the gynocardates, the hydnocarpate value being 

 at least twenty times that of the chaulmoograte. 



CONCLUSIONS 



1. Chaulmoogra oil and its derivatives exert a pronounced 

 growth-inhibiting action on Bacillus tuberculosis in vitro. 



2. This inhibition is specific ; that is to say, it is noticeable in 

 dilutions of the oil in which no inhibition of non-acid-fast bacilli 

 can be discerned. , 



3. Oils obtained from plants related to Taraktogenos kurzii 

 have a property similar to that of chaulmoogra oil with regard 

 to Bacillus tuberculosis. Hydnocarpus wightiana, H. alcalae, 

 H. subfalcata, and#. venenata, all containing the optically active 

 acids, show antiseptic power in vitro, the strength of the various 



'For details see Philip. Journ. Sci. 21 (1922) 1-15. 



