23,6 Perkins and Cruz: Oils in Chaulmoogra Group 557 



distillation was made of each, and four out of the six fractions 

 were examined, each of the four being given a single crystal- 

 lization. The separations are incomplete, but data have been 

 obtained which already have a comparative value and will have 

 a quantitative value when the composition of any one of the 

 oils becomes known. 



A mixture of 500 grams of the oil, 750 mils of 95 per cent 

 alcohol, and 20 mils of sulphuric acid (specific gravity, 1.84) 

 was put in a round-bottom flask fitted with a reflux condenser 

 and boiled vigorously for sixteen hours. The resulting crude 

 ester mixture was washed with water, and then with dilute 

 sodium hydroxide, making sure that a slight excess of sodium 

 hydroxide remained after thorough shaking in the cold. The 

 emulsion was broken up by heating, and the separated ester 

 was thoroughly dried and weighed. The acidity of the dried 

 ester mixture was then determined. (The weight should be 

 at least 450 grams and the acidity not more than 0.2 per cent.) 



Three hundred mils of the dried ester were distilled in vacuo 

 in a standard Pyrex 500-mil Hempel distilling flask, the neck of 

 which was filled with broken glass. Fractions of 50 mils each 

 were collected. The temperature limits and pressure for each 

 fraction were noted, and each was weighed. 



The first, second, fifth, and sixth fractions were separately 

 thoroughly saponified and the fatty acids set free. The freed 

 acids from each were recrystallized from 200 mils of 80 per 

 cent alcohol, cooling to about 15° C, and the filtrates again 

 saponified and treated with acid. The crystals are designated 

 (Tables 4, 5, 6, 7) by a, in addition to the appropriate fraction 

 number, and the fatty acids (completely free from inorganic 

 acid) from the alcoholic filtrates are designated by b. 



The weight, freezing point, iodine number, and specific rota- 

 tory power in xylene of each fraction were recorded. 



Fractions 3 and 4 of most of the oils were not tested, for 

 the reason that they are intermediate fractions and the data 

 gained do not appear worth the time consumed. The general 

 tenor of the data on these fractions may be seen in Tables 6 

 and 7, where they are recorded for a few of the samples. 



During the latter part of the work the pressure was regulated 

 at 20 millimeters by an adjustable mercury trap. 



