22, i Sellards and Leiva: Amoebic Dysentery 3 



to be tested were prepared with this solution and, without 

 sterilization, were immediately inoculated from a suspension of 

 amoeba from a twenty-four hour culture on agar. All incuba- 

 tions were made at 37° C. Observations were recorded at daily 

 intervals until encystment of the amoebae in the control cultures 

 had occurred. A repetition of all tests was made with freshly 

 prepared solutions. The results are recorded in Table 1. 



It is impossible to compare the data in Table 1 with the results 

 of other workers, on account of the variation in the species of 

 amoeba- that were employed. It is clear, however, that Vedder's 

 results are again amply confirmed. 



Quinine, used as the dihydrochloride, exhibited a pronounced 

 restraining effect on these cultures of amoebae; of the substances 

 tested it was second only to emetine. 



