22 The Philippine Journal of Science 1923 



November 11. Enema, negative. 



November 12. Nauseated, refuses food; suppression of urine; 200 

 cubic centimeters of normal saline injected intraperitoneally. 



November 12. Weight, 1,900 grams; appetite good; voids freely; enema, 



November 18. Weight, 1,950 grams; formed stool shows no blood nor 



November 20. Weight, 1,850 grams; freshly passed stool is negative. 



November 24. Found dead; several active ulcers in the intestine and 

 two amoebic abscesses in the liver; total emetine, 28 milligrams per kilo- 

 gram within five days. 



Of two control animals one, weighing 1,000 grams, showed 

 an incubation period of three days and died on the sixth day 

 after injection of amoeba- the other, weighing 1,450 grams, 

 had an incubation period of four days and died three days 

 later. 



In the next experiment one kitten and five adult cats were 

 injected with a patient's stool, rich in blood and mucus and 

 containing numerous active amoebae. None of the adults became 

 infected. 



STRAIN II ; FIRST PASSAGE ; CAT 2 



August 31 1921. Weight, 870 grams; inoculated direct from patient. 



beptember 6. Enema, abundant blood, mucus, and amoebae; incubation 

 r « n B w T'' Gmetine ' 10 ° Ubic centime ^rs per kilogram; an hour later 

 IT^rT^™ ^ ^^ Sh0Wing a f6W — — - T 



September 7. Weight, 820 grams; enema, no blood, mucus, nor amcebar 

 emetine, 10 milligrams per kilogram; two hours later a fsecal stool was 

 passed ; microscopically red blood cells were seen but no amceb* 



September 8. Weight, 870 grams; two soft f*cal stools" cage- enema 



ne fenirr e Q in % 5 mmigramS P6r kil ° gram -tramuscularly ' 



September 9. Emetine, 10 milligrams per kilogram. 



JZ^ZJLI^ 85 ° «*""> -^ f — d ** ■-*.; 



September 12. Fresh stool is negative 



September 13. Weight, 770 grams; no stools passed. 



September 15. Soft faecal stool negative for amoeba, 



shows no scars of healed lesions and microscopically no am^ ! ^T^ 

 from the heart blood showed a growth of staphylococcus- total emetinl ^ 

 milligrams per kilogram within three days. ococcus ' total emetine, 35 



In the following experiment, four adult cats (Nos 3 4 5 

 and 6) were injected with amoeba; two were treated, 'bacterial 

 cTtrols deVel ° pin * ver ^ ear *> ^d two were reserved for 



