22 ' 1 Sellards and Leiva: Amcebic Dysentery 23 



September 28, 1921. Weight, 1,830 grams; injected direct from patient. 



October 3. Enema, copious amount of blood rich in amoeba; incubation 

 period, five days; emetine, 10 milligrams per kilogram. 



October 4. Weight, 1,800 grams; stool of tarry consistency; no fresh 

 blood and no amoebae; emetine, 10 milligrams per kilogram. 



October 5. Weight, 1,720 grams; tarry stool shows no amoebae; emetine, 

 10 milligrams per kilogram. 



October 6. Enema, some blood and after long search one amoeba was 



October 7. Found dead; broncho-pneumonia; no inflammation nor ul- 

 ceration of the large bowel; total emetine, 30 milligrams per kilogram 



September 28, 1921. Weight, 1,500 grams; injected direct from patient. 



October 3. Enema, fresh blood showing many active amoebae; incuba- 

 tion period, five days; emetine, 10 milligrams per kilogram. 



October 4. Soft faecal stool, no amoeba?; enema, no blood nor amoebae; 

 emetine, 10 milligrams per kilogram. 



October 5. Weight, 1,400 grams; emetine, 10 milligrams per kilogram. 



October 6. Enema, no blood nor amoebae. 



October 7. Very ill; sacrificed; no pneumonia; one ulcer, 7 millimeters 

 in diameter, in lower third of large bowel; no amoebae found; blood culture 

 showed a staphylococcus; total emetine, 30 milligrams per kilogram within 

 two days. 



Control for Nos. 3 and 4. 



September 28, 1921. Weight, 1,850 grams; injected with amoebae. 

 October 3. Positive for amoebae; incubation period, five days. 

 October 4. Dead; typical ulceration of intestine. 



Control for Nos. 3 and 4. 



September 28, 1921. Weight, 1,740 grams; injected with amoeba?. 



October 4. Passing mucus containing a few amoebae; incubation period, 



October 7. Dead; typical ulceration of intestine. 



STRAIN III ; FIRST PASSAGE J CAT 7 



Delayed treatment. 



September 11, 1921. Weight, 800 grams; injected with amoebae. 



September 15. Passing blood rich in amoeba?; incubation period, four 

 days; no treatment. 



September 16. Bloody stools rich in amoebae; emetine, 10 milligrams 

 per kilogram; forty-five minutes later a stool was passed containing many 

 degenerating amoebae. 



September 17. Frequent bloody stools, numerous active amoebae; eme- 

 tine, 10 milligrams per kilogram. 



September 18. Found dead; extensive typical lesions of the lower third 

 of the large bowel; amoebae still motile; total emetine, 20 milligrams per 

 kilogram within one day. 



In the next experiment, four kittens were inoculated. All 

 became infected and three were treated. 



