22, i Sellards and Leiva: Amoebic Dysentery 25 



Of seven animals inoculated with Strain V in the second 

 passage, four became infected (Nos. 12 to 15) . 



November 13, 1921. Weight, 2,400 grams; injected with amoebae. 



November 15. Enema, trace of blood and several active amoebae; incu- 

 bation period, two days; emetine, 10 milligrams per kilogram. 



November 16. Weight, 2,350 grams; enema, no gross blood; micro- 

 scopically, a few red cells, no amoebae; emetine, 10 milligrams per kilogram. 



November 17. Weight, 2,320 grams; tarry stool; no amoebae; emetine, 

 5 milligrams per kilogram, expelled almost immediately; 2.5 milligrams 

 per kilogram repeated subcutaneously. 



November 18. Weight, 2,250 grams; enema, negative; emetine, 5 milli- 

 grams per kilogram returned at once. 



November 19. Weight, 2,270 grams; enema, negative. 



November 20. Weight, 2,150 grams; enema, negative. 



November 21. Ill; sacrificed; extensive broncho-penumonia on right side; 

 no inflammation of bowel, no amoebae found; total emetine, probably 25 to 

 30 milligrams per kilogram within three days. 



November 13, 1921. Weight, 650 grams; injected with amoebae. 



November 15. Enema, negative. 



November 16. Enema, abundant mucus and many active amoebae; in- 

 cubation period, three days; emetine, 10 milligrams per kilogram; con- 

 siderable loss after fifteen minutes; 2.5 milligrams per kilogram repeated 

 subcutaneously. 



November 17. Enema, negative; emetine, 2.5 milligrams per kilogram 

 subcutaneously. 



November 18. Weight, 570 grams; small bloody stool rich in amoebae; 

 emetine, 5 milligrams per kilogram; retained. 



November 19. Enema, negative; emetine, 5 milligrams per kilogram. 



November 20. Enema, negative. 



November 21. Ill; sacrificed; extensive bilateral broncho-pneumonia; 

 some hyperaemia of the lower portion of the large bowel; no amoebae found; 

 total emetine, not over 25 milligrams per kilogram. 



Control for Nos. 12 and 13. 



November 13, 1921. Weight, 1,820 grams; injected with a 



November 16. Passed abundant mucus; one amoeba found. 



November 21. No stools. 



November 22. Passing copious amounts of blood and n 



November 16. Enema, : 



