The Philippine Journal of Science 



Control for Nos. 20, 21, and 22. 



November 26, 1921. Weight, 510 grams; amoebae injected. 



November 27. Two soft mucous stools; no amoebae. 



November 28. Blood-tinged stool; one motile amoeba found in fifteen 

 minutes' search; incubation period, two days. 



November 29. Passing blood freely with many amoebae. 



December 1. Very ill; sacrificed; inflammation of rectum with one large 

 ulcer; blood culture, coarse gram-positive bacillus; one other control for 

 this series died the second day after injection of amoeba?; no lesions of 



PROPHYLACTIC TESTS WITH EMETINE PER RECTUM 



December 4, 1921. Weight, 520 grams; emetine, 10 milligrams per kilo- 

 gram; a half hour later, amoebae injected. 



December 5. Emetine, 5 milligrams per kilogram. 



December 6. Enema, negative. 



December 8. Enema, negative. 



December 10. Enema, negative. 



December 12. Enema, blood and amoebae; incubation period, eight days. 



December 13. Sacrificed; extensive inflammation of lower third of large 

 bowel; active amoebae; blood culture developed a staphylococcus; total 

 emetine, 15 milligrams per kilogram within one day. 



STRAIN VI ; FIRST PASSAGE ; CAT 25 



December 4, 1921. Weight, 650 grams; emetine, 5 milligrams per kilo- 

 gram; a half hour later, amoebae injected. 



December 5. Emetine, 5 milligrams per kilogram. 



December 8. Enema, negative. 



December 13. Enema, negative. 



December 17. Enema, negative. 



December 19. Found dead; typical inflammation in lower part of bowel- 

 total emetine, 15 milligrams per kilogram within two days. 



Control for Nos. 24 and 25. 



December 4, 1921. Weight, 700 grams; injected amoebaa. 

 three e Tys! r ? * EnemS ' ******** ^ "* * m<Bh *> incuba *™ period, 



December 12. Dead; typical lesions; two older cats weighing 1 650 and 

 1,700 grams were inoculated as additional controls for this'seS, but they 

 failed to become infected. ' y 



TREATMENT WITH PAPAVERINE AND QUININE 



Tests for toxicity. -The fatal dose of papaverine for cats is 

 usually stated to be 100 milligrams per kilogram of body weight 

 injected subcutaneously. Two adult cats were injected per rec 

 turn with papaverine hydrochloride in 2 per cent solution, using 

 50 mi ligrams per kilogram. On the second day this dosage was 

 repeated in one animal; the other was given 75 milligrams per 

 kilogram. Both became extremely drowsy, but recovered 



