22,i Embrey: The Antiscorbutic Vitamine 79 



For all the foods except persimmon, basal diet 1 was used, 

 which consisted of: 



Per cent. 



Whole oats 87 



Rice bran (tikitiki) 10 



Calcium chloride and disodium orthophosphate (equal 



weights) 3 



The mixture of the above was given ad libitum; and, in ad- 

 dition, 30 cubic centimeters of whole cow's milk, previously 

 boiled forty-five minutes, were given daily. 



For the persimmon, basal diet 2 was used, which consisted of : 



Percent. 



Whole wheat 86 



Yeast 2 



Wheat bran 3 



Butter 3 



Calcium lactate 3 



Sodium chloride 3 



In the case of control guinea pig 3P, orange juice was added 

 and the scurvy symptoms disappeared. Each animal was kept 

 in a separate cage. Each morning all remains of the basal diet 

 for the preceding day were removed, and a weighed amount 

 of the food under examination was put in the cage. After a 

 few hours, if the food was not eaten, it was fed to the guinea 

 pig by hand; then the basal diet was given ad libitum. The 

 guinea pigs were weighed twice a week, and a record of their 

 weights was kept. Autopsy was performed on all guinea pigs 

 that died before the termination of the experiment, to see 

 whether the typical scurvy symptoms were present. The ex- 

 periment in Manila was unfortunately of only nine weeks' 

 duration, and it would be advisable to continue this work in 

 order to determine the minimum amount of each food that must 

 be administered daily to afford complete protection from scurvy. 

 A group of control guinea pigs was given the basal diets with- 

 out any fresh fruit or vegetable. 



The Chinese persimmon was fed at the Union Medical College, 

 Peking-, and the experiment lasted twenty-four weeks. 

 CONCLUSIONS 



1. All of the control guinea pigs died of acute scurvy in from 

 nineteen to twenty-one days. 



2. Of the foods examined, pomelo, cucumber, chico, and guava 

 afforded the best protection from scurvy. In each case^ 10 

 grams of the food given daily were sufficient to protect "the 



