HO The Philippine Journal of Science 1923 



series of experiments than those recorded in this paper was 

 planned, but as no specimens of Ascaris vitolorum have been 

 found recently, either in Los Bafios or in abattoirs in Manila, 

 I desire to record the results that have thus far been obtained. 



Extracts that were used in the experiments described were 

 prepared from worms that were recovered during post-mortem 

 examination of calves that succumbed to ascariasis. Only living 

 specimens were selected for the purpose of preparing the ex- 

 tracts, and the specimens were thoroughly and repeatedly washed 

 in physiological salt solution in order to free their surface from 

 adhering intestinal matter. The worms were then dried with 

 filter paper, placed in a glass dish, and cut into pieces about 

 2.5 centimeters long. The cut-up worms were placed in a 

 desiccator containing calcium chloride, and after several days' 

 drying the parasite material was sufficiently crisp to allow pul- 

 verization. 



Extracts were prepared in physiological salt solution (0.85 

 per cent solution of sodium chloride) by suspending a certain 

 quantity of triturated worm material in a certain quantity of 

 salt solution, as noted in connection with each series of experi- 

 ments, and allowing the mixture to remain in a cool, dark place 

 for from two to three hours. The mixtures were then filtered, 

 or the fluid was removed by means ©f a syringe without filtration. 



Unless otherwise stated, animals were injected intraperito- 

 neal^, and the usual aseptic precautions were adhered to in the 

 course of these experiments. All animals used in the experi- 

 ments were full grown. 



RECORDS OP EXPERIMENTS 



The following extract was used in this series of experiments : 

 One gram of finely powdered worm material was suspended in 

 10 cubic centimeters of physiological salt solution. 



Experiment i.— One and five-tenths cubic centimeters of the 

 filtered extract was injected into guinea pig 1. About fifteen 

 minutes after the injection the animal gave evidence of distress. 

 The following symptoms were most pronounced : Severe scratch- 

 ing, marked trembling and excitation, and a rough coat. These 

 symptoms gradually increased in severity and were followed by 

 weakness in the legs, a tendency to fall down, and finally by 

 paralysis. The animal was kept under observation for several 



