22,1 Schwartz: Extracts of Ascaris vitolorum HI 



hours, during which period the symptoms did not subside. The 

 following day the animal was found dead. 



Experiment 2. — Guinea pig 2 was injected with the same 

 quantity of extract as was used in experiment 1. The appear- 

 ance and persistence of symptoms and the results were the same 

 as in experiment 1. 



The following extract was used in the experiments of this 

 series: One gram of powder was suspended in 15 cubic centi- 

 meters of physiological salt solution. 



Experiment 3. — Guinea pig 3 was injected with 1 cubic centi- 

 meter of extract. The symptoms were practically the same as 

 those noted in experiment 1. There was a period during which 

 the animal showed evidence of distress by running excitedly up 

 and down the table and emitting shrill sounds from time to 

 time. This was followed by a state of dullness and stupor, 

 and complete prostration. This animal gradually recovered from 

 the injection. It was kept under observation for several weeks, 

 but showed no further symptoms. 



Experiment U. — Guinea pig 4 was injected with a quantity 

 of extract equal to that used in experiment 3, but the extract 

 was heated to boiling point and then allowed to cool before it 

 was injected. The results were similar to those noted in experi- 

 ment 3. 



Experiment 5. — Guinea pig 5 was injected with 2 cubic centi- 

 meters of extract. It exhibited the same symptoms as guinea 

 pigs 3 and 4, but its reactions were more pronounced. This 

 animal was found dead the next morning. 



Experiment 6. — Guinea pig 6 was given 5 cubic centimeters 

 of extract by mouth. The animal became ill immediately after 

 the administration of the extract, ran about excitedly along the 

 table, squealed as if in pain, scratched its face violently, rubbed 

 its mouth with its forelegs, and exhibited other nervous symp- 

 toms. The animal eventually recovered. 



SERIES III 



The powdered-worm material that was used in this and in the 

 following series of experiments was obtained from the same 

 lot of dried worms as was that used in Series I and II. Several 

 months elapsed between the experiments in the first two series 

 and those in subsequent series, and during the intervals the 

 dried-worm material was kept in a tightly corked bottle in a 

 dark place. 



