112 The Philippine Journal of Science 1923 



The extract used in this series of experiments was made up 

 as follows : One-half gram of powdered- worm material was ex- 

 tracted in 10 cubic centimeters of physiological salt solution. 



Experiment 7. — Guinea pig 7 was injected with 2 cubic centi- 

 meters of the extract. About five minutes after the injection the 

 animal began to exhibit signs of uneasiness. Its fur became 

 rough, its movements were spasmodic, and it ran about on the 

 table very excitedly, emitting guttural sounds. Ten minutes 

 later it showed weakness in the legs, and shifted its weight 

 from side to side. Its eyes were only half open, and it fell 

 down from time to time. About thirty minutes after the in- 

 jection the guinea pig was completely prostrated. It remained 

 under close observation for seven hours and throughout this 

 period was very ill. Its respiration was rapid and shallow, and 

 it was feverish and extremely sensitive to touch. Frequently it 

 squealed as if in pain. The following day the guinea pig was 

 quiet but rather weak. It refused food and drink. Gradually, 

 however, the animal recovered fully from the effects of the in- 

 jection. 



Experiment 8.— Guinea pig 8 was injected with 3 cubic centi- 

 meters of the extract. The behavior of this animal was similar 

 to that of guinea pig 7, but the symptoms appeared more rapidly 

 and were more marked. This animal was very ill the day 

 after the injection, but it finally recovered. 



The extract used in this series of experiments, which were 

 performed about three weeks later than the experiments in 

 Series III, was similar to that used in experiments 7 and 8. 

 White rats were substituted for guinea pigs in experiments 9 

 and 10. 



Experiment 9.— Rat 1 was injected with 1.4 cubic centi- 

 meters of the extract. Ten minutes after injection the animal 

 became greatly excited, jumped up and down in the glass jar in 

 which it was kept under observation, and scratched itself rather 

 violently. About ten minutes later the rat was dull and list- 

 less, its fur was rough, its back was arched, its head drooping, 

 and respiration labored, and its body jerked involuntarily from 

 time to time. Forty-five minutes after injection the rat lay 

 on its abdomen. This animal was sick for an entire day, during 

 which it was kept under close observation. It remained list- 

 less and weak. The following day the animal was weak and 

 did not eat. It recovered from the effects of the injection in 

 two days. 



