M Read and Wang: Metabolism in China 129 



EXAMINATION OF URINES 



Methods used for making examinations were the following: 

 Total nitrogen, estimated by Folin's micro method. Micro-Kjeldahl 



with subsequent Nesslerization. 

 Urea, soy-bean extract was used. After the action of the urease the 



ammonia was estimated by Folin's colorimetric method. 

 Ammonia, by aeration method. 



Creatinine, by Folin's picric acid colorimetric method. 

 Uric acid, by Folin's method. 

 Chlorides, by Volhard's method. 

 Sulphates, by barium gravimetric methods. 

 Phosphates, by titration with uranium acetate. 

 Table 2. — Summary of urine analyses of convalescent patients. 



Bed No. 



i 



I 



j 



I 

 J 



I 

 I 



i 



| 



1 



! 



i 



i 



s 



i 

 I 



j 

 j 



2 



I 

 I 



| 



| 





i 



2. 123 



5 



h 



H ; 



...7-.i 



n 



1.324 



s 



s 



S; 









5 











10 



Averse... 



,.„,>, 



,.« 



4.08 | 0.967 



M* 





l.r„« 



2.48! 



Table 2 is full of interest as a study of low protein metabolism, 

 it being in accord with other recent work along these lines 

 where workers were able to subsist on as low an amount as 0.5 

 gram of nitrogen per kilogram of body weight, in wheat, maize, 

 and oats. See the work of Sherman, (13) Rose and Cooper, (12) 

 and many others. 



The volumes of the urine excreted were the same as a normal 

 western average. However, as the urines were collected in the 

 winter months, they do not represent an average annual figure. 

 In the summer time the volumes are extremely low. 



ANALYSES OF URINES OF NORMAL INDIVIDUALS ON A 

 NONFLESH DIET 



Subsequently it was thought advisable to analyze the urines 

 of four laboratory helpers who were in apparently good health, 



