240 The Philippine Journal of Science 1923 



centimeter gave complete fixation, but complete haemolysis oc- 

 curred with 0.01 cubic centimeter of serum. On March 2 the 

 reaction was negative with 0.1 cubic centimeter of serum. 



On March 9, 1922, tissue from a yaw was implanted in two 

 incisions of the skin about 0.75 centimeter apart. A week later 

 (March 16) these incisions showed nothing more than ordinary 

 healing. At the end of the second week however (March 23) 

 each of these incisions showed a broadened elevated ridge of 

 epithelium. One week later (March 30) these lesions were 

 beginning to dry and regress. They are illustrated in Plate 

 2, fig. 1. The Wassermann reaction at this time was negative. 

 At the end of the fourth week (April 6) these small lesions had 

 desquamated completely. The final observation was made on 

 July 20. 



The fourth and last case came to the dispensary on September 

 12, 1921. An active mother yaw was present on the left wrist. 

 According to the history it appeared eight months ago. There 

 are numerous secondary yaws over the forehead and neck, around 

 the eyes and mouth, on the right hand and wrist, over the scrotum, 

 in both popliteal spaces, and on the right ankle. Neosalvarsan 

 was injected on this date. September 21 the skin lesions are 

 only partially healed. A second injection of neosalvarsan was 

 given which resulted in complete disappearance of the skin 

 lesions. 



The Wassermann reaction on September 12 showed complete 

 fixation with 0.01 cubic centimeter of serum, and almost complete 

 with 0.005 cubic centimeter. On October 24 complete fixation 

 occurred with 0.1 cubic centimeter of serum, but haemolysis was 

 almost complete with 0.01 cubic centimeter. On February 17, 

 1922, the reaction was negative with 0.1 cubic centimeter of 



The inoculation with yaws was made on March 9, 1922, in two 

 incisions about 1 centimeter apart. At the end of the first week 

 (March 16) there was a slight suggestion of elevation of the 

 epidermis along the line of healing. At the end of the second 

 week (March 23) this elevation was definite, and a week later 

 (March 30) at the lower incision a small definite granuloma had 

 developed, 7 millimeters in its longest diameter and about 2 to 

 3 millimeters in height. It appeared rather inactive, as though 

 spontaneous regression was about to set in. A photograph was 

 taken (Plate 2, fig. 2) and the lesion excised for histological 

 study. A section through this tissue shows a polymorphonuclear 



