442 The Philippine Journal of Science 192s 



The precipitate removed by centrifuging was washed twice with 

 95 per cent alcohol, once with absolute alcohol, once with ether, 

 and then dried. Two hundred milligrams of the dried precip- 

 itate were dissolved in 10 cubic centimeters of water made 

 alkaline to litmus with sodium carbonate. A small fraction 

 remaining undissolved was removed by centrifuging. This 

 solution was injected intravenously into an adult dog weighing 

 4.2 kilograms, under light ether anaesthesia. 



Before injection, pulse was 110; respiration, 20; rectal 

 temperature, 39° C. 



11 a. m. Solution injected slowly. Began to heave during 

 injection. Shortly afterward pulse was weak and mucous mem- 

 branes pale. Came out of anaesthesia slowly. Bowel movement 

 consisted of hard faeces. Got up and moved position. Listless 

 and sick. 



12.30 p. m. In complete collapse. Did not resist changes in 

 position. Muscles flaccid. Had voluminous fluid stool con- 

 taining flakes of mucus. Profuse salivation. Pulse 90, respi- 

 ration 24, rectal temperature, 37.5° C. Had rigors with stiff 

 legs and neck. 



1.00 p. m. Dead. Had more fluid bowel discharges. Au- 

 topsy showed general splanchnic congestion, with intense, brick- 

 red congestion of duodenal mucosa, the intensity gradually 

 fading off about 2 feet below the pylorus. Lumen of small 

 intestine filled with bile-stained fluid and mucus. Colon pale 

 and filled with fluid. Liver congested. Gall bladder injected 

 and cedematous. Heart's blood clots very slowly. 



In another experiment 0.3 gram of dried precipitate from a 

 different case injected into a dog weighing 5.6 kilograms was 

 fatal in one hour, with symptoms and pathological changes the 

 same as those described in experiment 3. In a third, 0.4 gram 

 was injected into a dog weighing 6.6 kilograms followed by 

 similar symptoms but resulting in recovery. Sublethal doses 

 of several other preparations of dried alcohol precipitate were 

 used ; the estimated fatal dose varied in different ones, but was 

 about 0.1 gram per kilogram. 



In the following two experiments unboiled fluid was used, 

 and this seemed to be more toxic than fluids subjected to greater 

 heat. 



EXPERIMENT 4 



Fifteen hundred cubic centimeters of fresh fluid stools from 

 cholera patients, within two or three hours after passage, were 

 heated in flasks, immersed in boiling water, for fifteen minutes. 



