22,6 Lee: A Disease of Orange Fruits 607 



frequently to be seen in old spores. The size of the spores is 

 from 4.3 to 6.0 by 15.0 to 21.5 p. Morphologically, therefore, the 

 fungus agrees entirely with Gloeosporium foliicolum Nishida. 

 The fungus has been grown in culture side by side with a known 

 culture of Gloeosporium foliicolum obtained from herbarium 

 material of that fungus forwarded by Doctor Nishida. Upon 

 nutrient agar slants, potato plugs, poured plates, and orange- 

 wood plugs, no cultural differences are discernible. The Wase 

 rot fungus fruits several days more slowly than does the known 

 culture of GloeospoHwm foliicolum, but such a difference cannot 

 be considered as an essential distinction. 



A culture of Doctor Nishida's Gloeosporium foliicolum was 

 then used as the inoculum for tests on Wase fruits. These inocu- 

 lations yielded positive results in about 33 per cent of the tests, 

 the lesions corresponding entirely with the Wase spot disease as 

 it occurs in the field. These inoculation results, together with 

 the morphological and cultural similarity, would indicate that 

 the fungus causing the Wase rot is identical with Gloeosporium, 

 foliicolum Nishida. 



THE RELATIONSHIPS OF GLOEOSPORIUM FOLIICOLUM, G. LIMET- 

 TICOLUM, AND COLLETOTRICHUM GLOEOSPORIOIDES 



Anthracnose and wither tip of limes, lemons, oranges, and 

 pummelos ascribed to Colletotrichum gloeospopoides were re- 

 viewed and described from Florida by Rolfs. 8) He described 

 this fungus as attacking small, newly formed lime fruite, new* 

 formed lime twigs, mature lemons, newly formed twigs of ^lemon 

 and newly formed twigs of orange and grapefruit, but not the 

 fruits of orange and grapefruit. Clausen O) later has sb 

 wither tip of the lime and lemon was not due tc > Collet o tnchum 

 gloeosportoides, but to a species that is f^J^^J^. 

 phological and cultural characters and in P^W<* actm* s 

 he described this species as Gloeosponum l ^ etU ^.^^ 

 did not have the opportunity to inoculate young lime fruits, 

 although he states the opinion that the anthracnose of young lime 



