g-^0 The Philippine Journal of Science 



Inoculations upon mature lemon, sweet orange, and pummelo 

 fruits.— Inoculations in the incubator at 25 to 26° C. were then 

 made upon mature fruits of American-grown lemons, C. hmoma, 

 and Japanese-grown Washington navel oranges, C. sinensis, and 

 pummelos, C. maxima. Upon the pummelos (Hirado variety) 

 two different series of ten fruits each were tried; all resulted 

 negatively. The Washington navel fruits in two series of twelve 

 fruits each all resulted negatively. The American lemons in 

 a single series of twelve fruits also resulted entirely negatively. 

 Control inoculations on twelve Ikiriki Unshiu fruits resulted in 

 58 per cent positive cases. These data can best be seen in tabular 

 form as follows: 



Table 2.— Showing results of ivocidatic 

 sporium foliicohim on fruits in in 

 inoculated with one needle punctur 



From these results it would seem that the fungus is restricted 

 to the fruits of Citrus nobilis, and has no pathogenicity on fruits 

 of other species. 



The results from the foregoing series of inoculations in the 

 incubator were repeated under orchard conditions, using both 

 fruits and twigs. Since Clausen's opinion is that anthracnose 

 of young lime fruits is due to Gloeosporium limetticolum f em- 

 phasis was placed upon the inoculation of such young fruits 

 since the results would show definitely the similarity or differ- 

 ences between the Japanese fungus and G. limetticolum. The 

 results are shown in Table 3. 



These inoculations show clearly the pathogenicity of the Japa- 

 nese Gloeosporium on mandarin oranges and the entire absence 

 of pathogenicity on young lime fruits or mature limes, lemons, 

 or sweet oranges. Further inoculations were made on fifteen 



