22 
mass of cytoplasm. During the preparation for the next division 
the large central vacuole is rapidly formed. In Peperomia Sin- 
tensii (Brown, 1908) an evanescent wall appears in the first divi-_ 
sion only; in other Peperomia’s (Campsent, 1899; Jonnson, 1900) 
cell wall formation is wholly omitted, but in all these cases 
cytoplasm remains homogenous up to the third division (= first 
division of the megaspores). 
embryosac- 
mothercell 
daughter- 
cells 
megaspores 
5 )) vacuolation 
4 ye Beco 
for 
alls; vacuolation 
trasp. embryosac 
©. g. Peperomia). 
o walls; satasiation 
following second di- 
vis.; bisp. embryosac 
(e. g. Gunnera) 
walls; vacuo — 
"Glateiag first divi 
mono nif embry 
Fig. 2. Polarisation (vacuolation) as means of recognizing megaspores. it ee 
Thirdly we get results from the application of the hypothesis: 
Up till now vacuolation was entirely left out of consideration 
by all authors, and embryosacs were considered of identical 
development, whether showing vacuolation after the first or 
second or third division. So for instance any reasonable expla- 
nation of conditions in Gunnera with its seven fusing nuclei and 
in Peperomia with its eight fusing nuclei, was absolutely lacking. 
