go BOTANICAL GAZETTE Laugust 



teleutospore increases the number of sporidia produced and so 

 increases the chances of infecting the aecidium host the following 

 pring- The developmental stages in this case were worked out in 

 greater detail in connection with the formation of the second spore 

 produced from the basal cell. About the time the cell division for 

 the formation of the first spore is complete, a hyphal bud is pushed 

 up beside the stalk of the first spore. A nuclear division occurs in 

 the region of the neck of this bud (fig. g). The spindles are here so 

 placed that there can be no question but that a daughter nucleus 

 from each of the nuclei of the basal cell wall enter the bud, while the 

 other two daughter nuclei will remain in the basal cell. The bud 

 is separated from the basal cell by a wall, thus forming the second 

 teleutospore-initial cell. A simultaneous nuclear division follows 

 within this cell {jig. lo), and a cell wall cuts off the spore cell and the 

 stalk cell. That it is the upper one of these two cells which produces 

 the two cells of the teleutospore is shown in fig, ii, where the cell 

 wall is just forming, A third bud is formed at about this time and a 

 third spore is produced in the same manner as has just been described 



(fig. 12). 



While at work on the teleuto stage of Puccinia podophylli a further 

 interesting phenomenon was observed. The occasional occurrence 

 of trinucleated cells, together wath Blackman's account of migra- 

 tions of nuclei among vegetative cells in Puccinia poarum, suggested 

 that possibly nuclear migrations occur in the fungi, as well as in the 

 higher plants, as purely pathological phenomena. 



MiEHE (19) has shown that when the epidermis is stripped from 

 the leaves of Allium nutans the nuclei of certain cells become pointed 

 and finally a thin beak penetrates the wall into a neighboring cell. 

 The portion of the nucleus which has passed the wall enlarges to 

 form a highly refractive, densely staining vesicle. This vesicle 

 increases in size as the nuclear material finally passes over into the 

 foreign cell. Miehe has found cells containing as many as five 

 nuclei as a result of such migration. The explanation given is that 

 the condition is a pathological one in which the nuclei migrate as a 

 reaction to the wound inflicted in removing the epidermis. 



ScHURHOFF (26} found essentially the same phenomena occurring 

 in Iris germanica. He also mentions the formation of the vesicle 

 which has a marked affinity for the red of the triple stain. 



