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UNIVERSITY OF CHICAGO PRESS 



• • 



CHICAGO. ILLINOIS 



? 



An Indispensable Book for Students of Botany 



Methods in Plant Histology 



SECOND EDITION— ILLUSTRATED 



By Charles J. Chamberlain, Ph.D. 



Instructor in Botany in the University of Chicago 



THIS BOOK contains directions for collecting and preparing plant material 

 for microscopic investigation. It is based upon a course in botanical micro- 

 technique, and is the first complete manual to be published on this subject. 

 Free-hand sectioning, the parafifin method, the celloidin method, and the glycerine 

 method are treated in detail. In later chapters specific directions are given for 

 rnaking such preparations as are needed by those who wish to study the plant 

 kingdom from the Algae up to the flowering plant. Special attention is paid to 

 the staining of karyokinetic figures, and formulas are given for the reagents 

 commonly used in the histological laboratory. In preparing the second edition the 

 author has kept in view the advance in the science since the book first appeared. 

 Professor Klebs's methods for securing the various reproductive phases in the 

 Algae and Fungi have been outlined in a practical way, and in general much 

 more attention has been given to collecting material. New chapters deal with 

 the Venetian turpentine method, microchemical tests, free-hand sections, special 

 methods, and the use of the microscope, 

 enlarged the volume from i68 to 272 pages. 



These changes and additions have 



272 pp., 8vo, cloth, net, $2.25 ; postpaid, $2.39 



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THE UNfVEP.SITY OF CHICAGO PRES 



