1907] 



STEVENS— CHRYSANTHEMUM RAY BLIGHT 



253 





i 



Inoculation no. i: under the Involucre of the bud which was just 

 beginning to open; December 8, growth observed; December 18, 

 exhibited typical disease. 



Inoculation no. 2: in the axil of the uppermost bract, in needle 

 pricks, with agar; the bud above was just beginning to open; 

 examined December 8 and 18 with no indication of disease. 



Inoculation no. j; with mycelium upon agar upon the end of 

 the unopened bud; examined December 8, no growth; December 18, 

 typical disease. 



Fig, 13. — Four diseased heads produced by inoculation from pure culture. 



Inoculation no. 4: control cultures similar to no. 3 with sterile 

 needle; examined December 8 and 18, no growth. 



Inoculation no. j: at base of leaf; the tissue unbroken; examined 

 December 8 and 18, no result. 



Inoculations nos. 6, 8: agar laid upon the outside of the involucre; 

 tissue uninjured; examined December 8 and 18, no results. 



Inoculation no. 7; lost through accident. 



Inoculation no. g: at the base of the petiole; tissue uninjured; 



examined December 8 and 18, no result. 



Inoculation no. 10: leaf base pricked and inoculated with agar 



■f 



culture; examined December 8 and 18, no results. 



It is to be noted that in this series nos. i and 3 produced typical 

 disease; that is, all of the inoculations which were attempted within 



