254 BOTANICAL GAZETTE [October 



the involucre. Control inoculations produced no growth, and 

 attempts to produce the disease upon leaves and stems through 

 uninjured tissue failed. 



Series II 



mvcelium 



set with pycnidia. 



Inocidations nos. ii^ 12: agar bearing mycelium placed deep 

 between the petals of the open flower; examined December 8, show^ed 

 typical disease. 



Inoculation no. ij: same as no. 11 using sterile agar; control 

 inoculation; examined December 8 and 18, no growth. 



Inoculation no, 14: peduncle immediately below the bud scarified 

 and inoculated with mycelium on agar; examined December 8, 

 typical disease. 



Inoculation no. 15: similar to no. 14, but with sterile agar; con- 

 trol experiment; examined December 8 and 18, no grow^th. 



Inoculation no. 16: upon scarified stem immediately under head 

 with agar bearing mycelium, the whole covered with wet cotton; 

 examined December 8 and 18, no result. 



Inoculation no. ly: conditions same as in no. 16; examined 

 December 18, head typically diseased. 



Inoculation no. 18: conditions same as in no. 16; examined Decem- 

 ber 8, no result; December 10, typical disease. 



Inoculation no. ig: 



m 



of the opening head; examined December 8, no growth; December 

 185 typical disease. 



Inoculation no. 20: conditions same as in no. 19; examined 

 December 8, typical disease. 



Inoculation no. 21: conditions same as in no. 19; lost through 

 accident. 



It is to be obsen^d that in this series the inoculations within the 

 flower produced typical disease. Control inoculations produced 

 no disease. Inoculation upon scarified stem when covered w^ith 

 damp cotton to prevent evaporation w^as capable of inducing the 

 disease. 



