256 BOTANICAL GAZETTE [October 



Series III 



Inoculated November 29, 1906. 



Inoculation no. 22: agar bearing rnycelium was placed between 

 the rays of a head and the whole covered with a tube and supplied 

 with wet cotton to prevent evaporation; tube and cotton removed 

 next day; examined December 8, typical disease. 



Inoculation no. 2j: similar to no. 22 but inoculation made just 

 inside the involucre; examined December 8, typical disease. 



Inoculation no. 24: similar to no. 22 but upon the tip of the bud; 

 covering tube removed after two days; examined December 8, typi- 

 cal disease. 



Inoculation no. 2j: a single ray from a diseased flower placed 

 among the rays of a flower just beginning to open, which w^ere left 

 uncovered; examined December 8, typical disease. 



Inoculation no. 26: similar to no. 25; but covered wnth tube as 

 in no. 22; examined December 8, typical disease. 



Inoculation no. 2j: similar to no. 22; examined December 8, 

 typical disease. 



From inoculations nos. 22, 23, 24, and 27 it seems evident the 

 protection of the inoculations from evaporation for 24 or 48 hours 

 leads to greater certainty and rapidity of infection. Inoculations 

 nos. 25 and 26 go to show the lack of necessity for any covering when 

 diseased rays are used with which to inoculate. It is possible that 

 the mycelium is in a more vigorous condition in such diseased rays. 



Series IV 



Inoculations made November 30, 1906, from very vigorously 



m from 



from 



taken from the edge of the colony; inoculation under the outer rays; 

 examined December 8, no growth; December 18, typical disease 

 in all. 



material 



dense zone in the colony; inoculated as in nos. 28 to 31; examined 

 December 8; no growth; December 18, ah typically diseased. 



Inoculations j6 to 40: inoculating material taken from the center 



