I 



1907] 



OSTERHOUT— BALANCED SOLUTIONS 



263 



The gemmae were removed from the cups with the point of a 

 knife and sprinkled on the surface of the solution, care being taken 

 to exclude particles of dust and dirt. Each tumbler contained 200^^ 

 of solution and was covered with a glass plate. The gemmae may 

 be easily removed from the surface of the solution for purposes of 

 observation by dipping into it a clean slide and slowly raising it at an 

 angle so as to take up the material upon one side only. The material 

 should not be replaced in the solution unless extreme care be taken 

 to prevent contamination. 



The average results of six series of experiments are given in Tables 

 IV and V, and illustrated in figs. 2 and j. 



k 



TABLE IV. LUNULARIA 



A plus sign indicates that the plants were alive when the experiment was stopped 

 All quantities given are cubic centimeters of 3W/32 solutions. 



Culture Solution 



Duration of Life in Days 



Dilute sea water; total salts 



0.6 per cent , 



Dilute artificial sea water: 

 1000 NaCl 

 78 MgCU 

 38 MgS04 

 22 KCl 



10 CaCU 

 Distilled water. . . . 



• NaCl 



1000 NaCl 



10 CaCl 



rooo NaCI 

 22 KCl 

 10 CaCIj 



1000 NaCl 



78 MgCU 

 10 CaCla 



1000 NaCl 

 78 MgCU 

 22 KCl 



1000 NaCl 

 22 KCl 



1000 

 78 



NaCl 

 MgCl. 

 MgCl 

 KCl . 

 CaCU 



200-1- 



200-f 



200 + 

 4 



100 



200 + 



200 -i- 



9 



4 



7 



2 



12 

 100 



^m- 



