266 



BOTANICAL GAZETTE 



[OCTOBER 



wheat and other small seeds during the first stages of germination; 



but if the experiments are to be carried beyond this stage, the seed- 

 hngs must be supported so that the leaves do not come into contact 



with the solution. After 



I 



2 



o 



6 some trials the following 

 device was hit upon which 

 answers the purpose 

 admirably. A strip of 



filter paper is 



folded 



and one of 



the folds turned back as 



lengthwise 



shown in fig. 5 



The 



seeds are placed in the 

 trough thus formed and 

 the whole strip is then 

 bent into a circle and 

 placed in a tumbler previ- 

 ously filled with solution. 

 The strip should be of 

 such length that when 

 placed in the top of the 



Fig. 4,— Development of spores tumbler the ends jUSt 



meet and so form a stiff 

 collar which just fits 

 inside the top of the 

 tumblers and which will 

 not slip down. A large 

 number of these collars 

 may be prepared, filled 

 with seeds, bent into 

 circles, and secured by 

 ordinary paper-clips 



of Equisetum in various 3W/160 

 solutions during 50 days. Quanti- 

 ties are stated in cubic 

 meters; the gain 

 thallus exclusive of 



centi- 



in length of 



rhizoids is 



I, distilled 



stated in per cent, 

 water, gain 1760. 2, dilute arti- 

 ficial sea water, NaCl 

 3w/i6o, gain 1500. 3, 

 1000 + KCl 22 + CaCU 10, gain 

 1500. 4, NaCl looo+CaCla lo, 

 gain 980, 5, CaCU, gain 700. 

 6, NaCl, gain o. 



= ap. 

 NaCl 



placed on the overlapping ends- 



wanted. 



may 



only 



collars in glasses previously filled with solutions. 



In some 



where larger glasses are employed, a 



may 



makes 



( 



