; 



1907] 



OSTERHOUT— BALANCED SOLUTIONS 



267 



4 



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i 



collar. It is then advisable to perforate the bottom of the seed trough 

 by means of a tracing wheel such as is used for patterns. This allows 

 the roots to penetrate the paper freely and without delay. 



Care should be taken that the solution does not cover the seeds. 

 The paper must be spread open at the top so as to allow the air to 

 come into direct contact with the seeds. The micropyle should be in 

 contact with the moist filter paper. 



Careful experiments were made to determine whether the filter 



r 



paper exerted any influence on the solution (by absorption, etc., 

 or by concentration of the solution about the seed as the result of 

 evaporation) which might affect the results, but no such influence 



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I 



TABLE VI. EQUISETUM 

 All quantities given are cubic centimeters of 3^/160 solutions. 



Culture Solutiox 



Growth in 50 Davs 



Length of thallus 

 in mm. 



Dilute artificial sea water; 

 1000 NaCl 



38 MgS04 

 22 KCl 

 10 CaCi:^ 

 DistiUed water. . , . 



NaCl... 

 1000 NaCl 

 10 CaCU 



1000 NaCl 

 22 KCl 

 10 CaCU 



1000 



78 

 10 



Nad 



MgCl, 



CaCl, 



1000 NaCl 



78 MgCU 

 22 KCl 



1000 NaCl 

 22 KCl 



1000 



78 



NaCl 



MgCU 



MgCU 

 KCL. 



CaCU. 



0.80 



0-93 

 0.05 



0.54 



0.80 



0.93 



0.07 



0.05 



0.07 



0.05 

 0.05 



0.40 



Per cent, increase in 



length of thallus. 



exclusive of 



rhizoids 



1500 



1760 



o 



980 



1500 



1760 



40 



40 



o 



o 



7CXD 



Aggregate length 

 of rhizoids per 

 thallus in mm. 



8.1 



9.0 

 o 



4.7 



5-^ 



9.0 



o 



o 



3-2 



