12 BOTANICAL GAZETTE. [ January, © 
turpentine, which soon entirely dissolves the paraftin. The 4 
sections now stick firmly to the glass and the slides can pass — 
through various liquids without the sections being detached, — 
After having remained for some time in turpentine, this is — 
washed out with alcohol of 95 %. ’ 
€ now proceed to staining the sections, but I can not — 
enter into many details here, which are to be sought for in : 
works treating of microscopic manipulatioa. 
only observe that sections made in the manner here de- 
scribed require to be stained in order fully to enjoy the ad- 
vantages of the imbedding method. : 
will add some directions as to the staining of the speci- ; 
mens which we have here chosen as an example, viz., the 
roots of Viciaor Allium. These roots may be stained before — 
imbedding, but in this case it will be preferable to use roots 
which have been treated with picric or chromic acid instea 
of Flemming’s Mixture. 
I put them for twenty-four hours into a solution of Gren- 
acher’s alum-carmine after they have reached the alcohol 0 
#. After this they come again into alcohol and are 
further treated as described above. When the paraffin has 4 
been dissolved out of the sections by means of turpentine, it _ 
may be directly replaced in this casé by a mounting medium: 
oil of cloves, Canada balsam or glycerine, the latter after 
try the imbedding method. In the case of a single root 
many slides may be obtained, and thus it will be easy to try 
alum-carmine, in which the slide 
to twenty-four hours. With haematoxylin a similar effect is— 
obtained, and if this should be applied at a temperature of 
50° C. the process of staining will only take from ten to 
twenty minutes.’ With both these dyes the protoplasm, 
whieh wiht fi prmula for obtaining in a few hours a sol matoxylin- 
se very long time without ¢ reci - As if 
givenina dissertation; written in the Dutch langu ore tere iH ‘ 
et as . bi. This aud ~* me 
: Closed giass vessel at a temperature ee: 
and n filtered it is immediately fit for use. Some carton acid aided 
to it, and for s it is D. G. Siegenbeek von Hen 
