ST eT ee Oa ee en ae Re ee ee ee ee =" 
1888. | BOTANICAL GAZETTE. 13 
and especially the nuclei, are beautifully colored, and the 
colors of the cells will be plainly discernible, so that it will 
be a very easy matter to make an exact drawing of the whole 
twenty-four hours, or at a temperature of 50° C, for a much 
shorter time (one hour). After this they are treated for some 
seconds with absolute alcohol, containing $% or less of hydro- 
chloric acid, then washed out well, first in water with a tew 
rops of ammonia, g. 10 drops in 300 cub. cent. of 
water), afterwards in neutral alcohol. Finally, the sections 
are mounted in oil of cloves, and afterwards in Canada bal- 
sam. Successful preparations of this kind will exhibit most 
beautifully the longitudinal division of the segments Into 
which the nucleus is dissolved. 
gentian-violet, or as Dr. H. Twaardemaher recommends - 
To a concentrated watery solution of anilin-oil is added 
ity of 
of safranin. In this liquid the slides remain for 
