32 REPORT OF THE COMMISSIONER OF FISHERIES. 
the Bureau has been successful in the penning of the Pacific coast sal- 
mon for an extended period, but it must be borne in mind that the 
water at Baker Lake is always at a much lower temperature than the 
water at any other station where salmon operations are conducted. 
The method of killing and bleeding the fish by cutting off their tails 
before taking the spawn has been adopted at this station, and the use 
of a normal salt solution for washing the eggs has not been found 
necessary if the fish are properly bled. 
The method of taking spawn at the Clackamas hatchery and its sub- 
stations was similar to that of previous years, but several experiments 
were tried to test the efficacy of bleeding the fish prior to taking the 
egos, and the adyantage of this method, if any, over the use of a normal 
salt solution for washing the eggs. Experiments were also made to 
determine whether or not eggs should be washed before they are trans- 
portedy A million eggs were taken by killing the fish and extrud- 
ing the eggs by hand pressure; the eggs were then washed and ferti- 
lized, and they hatched with a loss of 10.6 per cent. Six hundredand 
eleven thousand eggs were taken by killing the females, bleeding by 
cutting off the tail, pressing the eggs out by hand, and washing them 
with a normal! salt solution. This lot hatched with a loss of 18.7 per 
cent. Two million six hundred and fifty thousand eggs were taken by 
killing the fish, bleeding them by cutting off the tail, pressing the 
egos out by hand and fertilizing without washing. These hatched 
with a loss of 9.9 per cent. Seven hundred and fifty-four thousand 
egos were taken from fish which were killed and not bled, the eggs 
being taken by incision and washed ina normal salt solution before 
being fertilized. The loss in this case was 3.8 per cent. Two million 
five hundred and ninety-three thousand eggs were obtained by killing 
and bleeding the females, then taking the eggs by incision and washing 
without the use of the normal salt solution. The loss in hatching 
amounted to 1.5 per cent. Six hundred and nine thousand eges were 
taken by killing and bleeding the fish, taking the eggs by incision, 
and washing ina normal salt solution. These hatched with a loss of 
2.02 per cent. One hundred and seventy-six thousand eggs were 
taken by incision after killing and bleeding the fish, and washed in 
a normal salt solution. These hatched witha loss of 1.9 per cent. 
The experiments were not concluded. 
A large number of young salmon, the product of eggs obtained at 
various substations, were reared to the fingerling stage and marked 
before being liberated. The adipose fin was removed on all, and in 
order to identify the different lots the fish hatched at Clackamas were 
given an additional mark by removing the anterior portion of the 
dorsal fin. The posterior half of the dorsal fin was removed from the 
fish produced at Little White Salmon, the anterior half of the anal 
