262 ; BOTANICAL GAZETTE [APRIL 
osporium from the cranberry, Vaccinium macrocarpum; G. elasticae Cooke & 
e, from the leaves of the rubber plant, Ficus elastica; a form from the 
locust, Gleditschia iriacanthus, which does not appear to have been reported 
ore; one from Ginkgo biloba, also not heretofore sd. Colletotrichum 
. Southw., from cotton; and C. Lindemuthianum (Sacc. & Magnus) 
& Cav., thos the cultivated bean. 
Of these eight only the one from the apple has had its ascogenous 
form reported heretofore. Miss SroneMAN worked with the anthrac- 
nose of the bean, but was unable to produce the ascogenous stage. 
All the forms mentioned have been grown by the writers in pure 
cultures, and the ascogenous stages produced, in most cases, several 
times. The original cultures were usually obtained by the transfer 
to culture media of conidia or portions of the tissue of the host con- 
taining the hyphae of the fungus. We have been unable as yet to 
tell definitely what the factors are which control the production of 
the ascogenous form. Repeated experiments with various culture 
media, under various conditions of light, temperature, and moisture, 
seem to indicate that these are not factors of great importance. Once 
having obtained a race, strain, or generation which produces ascoge- 
nous perithecia in cultures, it can be successfully grown on various 
media and under various conditions for several generations. 
The medium which we have found best adapted for use and which 
has produced the most abundant and luxuriant growths of ascogenous 
forms is sterilized corn meal. We have found by repeated experi- 
ments that if a culture from any particular acervulus or group of 
acervuli does not produce an ascogenous stage on corn meal at 75° 
to 85° F-., it is useless to experiment further with material from the 
same source. Fresh material from other specimens must be tried - 
until a race, strain, or generation is found which will produce its 
ascogenous form. It has been suggested that the nearness or remote- 
ness of origin of the conidia from ascospores may be an important 
factor in determining the production of an ascogenous stage. The 
few experiments we have made along this line are not conclusive, 
and more data must be obtained before results can be reported. 
Heretofore forms occurring on different hosts have been generally 
regarded as distinct species. The study which we have made of the 
eight forms just mentioned leads to the conclusion that they cannot 
