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1901 | GAMETOGENESIS AND FERTILIZATION IN ALBUGO 89 
with and even penetrate the coenocentrum (jigs. 32, 34), and 
are thus found during all stages of the second mitosis. In 
some cases only one nucleus in mitosis is found thus attached; 
in others as many as three seemed to be so anchored, and prob- 
ably, if the sphere could be viewed from all sides, as many as six 
or seven nuclei could be seen attached to the coenocentrum. 
The other primary oospheric nuclei are found lying free in the 
neighboring ooplasm in a similar stage of mitosis. It is not 
probable that any nuclei actually enter the coenocentrum, inas- 
much as it maintains its homogeneous appearance until consid- 
erably later. 
After the completion of the second mitosis, one small 
nucleus is found lying very close to the coenocentrum, possibly 
attached to it, although the evidence in A. Tragopogonis is not 
clear. Older stages show a larger nucleus. As the size of the 
nucleus increases, the coenocentrum becomes more granular 
( figs. 35-37), and loses its definite form, eventually appearing 
simply as a granular mass partially enveloping the female 
nucleus. As has been said, there is usually only one nucleus 
lying beside the coenocentrum after the completion of the 
second division, although several are in contact with it during 
this process. The oosphere when ready for fertilization contains 
one large nucleus which lies beside the remains of the coeno- 
centrum. <A few small degenerating nuclei, frequentiy present, 
are scattered throughout the ooplasm, which still maintains that 
fine-meshed alveolar structure that characterizes it after zona- 
tion. 
Few phenomena of interest were observed in connection with 
the entrance of the antheridial tube. It has a much thinner 
wall, and is of more gelatinous nature, than that of A. But or of 
A. Portulacae, and disappears more quickly after discharging its 
contents. It usually bears several degenerate nuclei as well as 
one or two that are larger and apparently capable of functioning. 
Fig. 33 shows an unopened tube which contains a greater num- 
ber, although in this case the oosphere clearly possessed only 
one functional nucleus. The rupture of the tube, accompanied 
