186 BOTANICAL GAZETTE [SEPTEMBER 
after the appearance of the proteid matter in quantities that 
obscured the other cell features, cyanin and erythrosin, after 
treating the sections on the slide with dilute sulfuric acid and 
chloroform, produced very satisfactory results. The power to 
take up the stains was retarded in the case of the proteid 
granules, whereas the nuclei and cell walls were more readily 
and more brilliantly dyed after this treatment. Karyokinetic 
figures were especially clear. 
It remains my pleasant duty to express my thanks to Dr. 
John M. Coulter, and to other members of the Botanical Depart- 
ment of the University of Chicago, for many suggestions 
offered in the course of this investigation. To Mr. E. J. Can- 
ning, Head Gardener of the Botanic Gardens of Smith College, 
I am deeply indebted for aid in collecting and growing 
material. 
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