318 BOTANICAL GAZETTE | NOVEMBER 
green color, filled with spherical bodies (fig. 73), which in gen- 
eral resembled a sporangium. But while these cells were 
observed daily for weeks they not only failed to show any signs 
of further development, but gradually disintegrated instead. 
After having observed algae which were crushed and noting 
the manner in which the chromatophores formed a colorless 
membrane about themselves, with the green collecting at one 
side, as in fig. 77, it would seem almost certain that the con- 
ditions I had been observing were due to injury, and the admis- 
sion of a small amount of water caused the abnormal appearance 
suggesting spores. On one occasion, a plant of this sort showed 
four or five biciliate organisms within it, which at first were sup- 
posed to be zoospores (fig. rg). Their subsequent development 
(fig. 15), however, proved them to have no connection with the 
alga, and after three days, when the culture was crushed out, it 
was found that the organisms possessed no chlorophyll, a fact 
which could not be made clear with certainty while they were 
embedded among the chromatophores. 
Failing to find zoospores under natural conditions, it was 
hoped that some change in the environment might be produced 
which would cause zoospore formation. Plants, closely encysted 
in numerous gelatinous coats and which had been growing in a 
nutrient solution, were removed to fresh water and to variously 
modified media. Some cultures were allowed to remain in the 
sunlight, others were kept in darkness. Specimens taken direct 
from nature were placed in numerous solutions and subjected to 
all possible changes of temperature and moisture. Aeration was 
tried for lengths of time varying from a few hours to several 
weeks, and cells were kept on ice over night, and then gradually 
brought into the temperature of the laboratory. But none of 
these methods yielded any thing more than the previously des- 
cribed method of division. Consequently, after having had 
cultures running for over three years, and making repeated 
examinations of material collected under natural conditions, it 
must be said that at no time was there any appearance which 
gave the slightest evidence of zoospore formation, and it 
