BOTANICAL GAZETTE, 305 
spore. In the vegetative cell (c) is seen a constant result of the 
formation of a zygospore in a contiguous cell. In the fertilized 
cell (a) pressure seems to have been removed sufficiently to allow 
the pressure in the vegetative cell (c) to push outward the inter- 
vening partition, just as happens to a partition when a contigu- 
ous cell has been ruptured. 
Figure 2. Occasionally two zygospores are found in the same 
cell, formed by the one act of fertilization, but usually somewhat 
smaller than those in adjoining cells. 
igure 3. Between the cells a and a’ the usual conjugating 
tube has developed, and doubtless there has been a certain union 
of the contents, yet in each cell there isa perfect zygospore. They 
are not quite so large as those of adjoining cells. This condition 
of things was found as often as three times in the same pair of 
filaments. The bulging of the cell ¢ into a’, owing to a relief of 
pressure in the latter, is apparent, but becomes very noticable in 
the next figure. 
Figure When a filament has broken up into unusually 
short cells and then conjugates with a large-celled filament, there 
is not always room for the zygospore in the female cell, and sue 
distorted forms as those shown in the figure are the result. In 
this case the bulging walls of the vegetative cells have still fur- 
ther diminished the space. : 
Figur The dumb-bell zygospore of this figure could not 
be explained, unless some such condition as in Fig. 1 shaped it 
until it became rigid. But of this there was not the slightest in- 
dication eg be 
Figure 6. While in general terms in the case of conjugating 
filaments one may be called fertile and the other sterile, as ex- 
pressing the usual direction of flow, yet zygospores are repeatedty 
found in the latter. In this figure there are four contiguous fila- 
ments. The first cell of 6 has been provided for by a, hence the 
first cell of ¢ is compelled to send out a very long tube towards 
- But the contents of ¢. have already assumed the zygospore 
sab W. hile d still has its spiral band, as though the latter was too 
ong in being reached. pl a 
igure. In the two filaments represented conjeEre, re 
already taken place between some cells, and is being prepare wai 
between aand b, yet a is a single banded cell, while in b there 
are two bands, So far as could be determined these two filaments 
“epresent two species, based upon the number 0 spiral bands. ‘ 
__» gure 8 shows an unusually great difference in the size 0 
Sonjugating cells, 
