344 BOTANICAL GAZETTE. 
in water,’ or by simply transferring a minute portion of the ex- 
udation from the diseased to the healthy shoot. The problem 
was consequently narrowed down to one of two alternatives, 
either the bacteria were the cause of the disease, or the juices 
which accompanied them were the cause of it. Bs, 
The first attempt was to secure some inoculating material in 
which the bacteria were entirely freed from the juices of the dis- 
ease. ‘This was done by means of a succession of artificial cul- 
tures in a sterilized infusion of corn (maize) meal. 
Wo series were successfully carried through, extending over 
about four months, and an inoculation from the sixth culture of 
each introduced into the green fruit of a Bartlett pear. 
€ accompanying tables will show the kind of culture ves- 
sels used, the amount of culture fluid they contained, and the 
date at which each one. was started. The first culture of the 
ee ee ee 
t of 
Soltis: ante ha Kind of Culture Vessel. ae 
i oe 
301 March 27 ..... Salmon culture tube 20 ce. 
302 | April 1......... Salmon cultare tube 20 cc. 
307 April 21........ Sternberg culture flask “ 3 ct. 
309 April 34... 4... Sternberg culture flask . 3 ce. 
314 May 22......... Stercberg culture flask oe 
322 June 5......... Test tube with Fol stopper.-..-.++++++++| 25 ce. 
364 | July 13.........) Bartlett pear on tree 
ee [oe 
series was infected with a very small fragment of wood taken 
from the inner portion of a diseased limb of Flemish Beauty 
pear, in which the disease had been slowly advancing during the 
winter, from an inoculation made July 26, 1884, with an infusion 
of blighted twig from an apple tree. The infection of each of - 
ee 
ount of 
cali set Sexi Kind of Culture Vessel. a eid. 
ee oe 
ads March 27...... Salmon culture tube..... scscccecesereee: i 
307 roe ae almon culture tube.......sessseeeereeeee? a ye 
309 pril 21 f ternberg culture flask. ......-++ +++ = 
April 24........ Sternberg culture flask.....-..+...::e+++ : 
— April 27........ Sternberg culture flask. .....+++++++++° Me, a a 
320 June 5 ........ ‘est tube with Fol stopper «--++-++++++++ 
36 July BB Sede Bartlett pear on tree 
ee a ee a 
is ic. Exper 
Station ey Bull. N. Y. Agric. Exper. Station, xcii: 34 Ann. Rep. N. Y. Agrie Le. 
* Burrill, Amer, Ass, Ady, Sci., xxix, p, 589; Amer. Naturalist, xv. 529. Arthur, * 
