BOTANICAL GAZETTE. 353 
The pollen-spore possesses two nuclei, one of which, the 
smaller, persists and either with or without division copulates 
with the female pronucleus. “ 
he pollen tube penetrates between the cells of the stigma 
ae passes down the conducting tissue and not in the-canal of 
the style. 
There is the usual generative and vegetative apparatus in the 
embryo-sac. . 
Mernops.—For the study of the development of the pollen- 
spores I used alcohol-fixed buds, which had been twenty-four 
hours in alcohol-glycerine®’, commencing with those 2mm. in 
length. The sections of the entire bud were stained with methyl- 
blue.’ The plant is an admirable one for the use of students in 
this respect. ‘ 
_ For the study of the pollen-spores themselves fresh material 
is requisite. The best results were obtained by staining with 
rax-carmine.” The spores are placed in a drop of 2 per cent. 
acetic acid and after a few minutes a drop of borax-carmine 
added. This is allowed to remain an hour, the slide being el 
tected from evaporation meanwhile. The stain Is then wash 
Tee Ree ee ce a . 
Te he eper pe ee si ey oo ee 
The spores were germinated in a hanging drop f fihgs a 
eent. sugar solution in the usual moist chamber. er th 
glycerine is — under the cover. Both yield sohcspae pipe 
:. oa in the tubes are thus more deeply stained tha 
*ytoplasm. P 
Longitudinal sections of the stigmas serve for ~ a: i 
the entrance of the pollen tubes. I used alcoholic m ; 
without any staining, mounted in glycerine. . 
. he pollen Fe Ba in the conducting tissue may be eae 
either ix longitudinal sections of the style or by aye . out 
ee and drawing a needle through the canal, thus tig ‘ge . 
the Conducting tissue. In the latter case care must be take 
