354 BOTANICAL GAZETTE. 
in picric acid” and in chrom-acetic acid" was used. e contrac- 
tion of the contents of the embryo-sac is unavoidable. I think 
the alcoholic material is quite equal to the others and less trouble- 
some. I found it necessary to depend on getting chance sections 
of the ovules by cutting the whole ovary longitudinally and ye 
ing the sections in glycerine. Previous to the cutting the 
material is placed in alcohol-glycerine for twenty-four hours or 
more. After 
clearer and clearer. I also tried cuttin sections in Mer i 
known directions by imbedding the ovules in colored pith to 
render them more easily seen. The results, on the whole, are 
not better than by depending on chance sections, and they ai 
much more troublesome. 
Seem eer ins ST 
Saturated aqueous solution. 
16 Chromic ecia, 0.7; acetic acid, 0.3; distilled water, 99. Strasburger, l. c., p. 328. 
plane ; the stigmas unopened and the style laden with pollen. aie 3, a style 
i 0 : 
. . * * . Fig. 5, 
with stigmas n - 4, interlocking papille of stigmas. : 
section of style from bud, ‘showin fe ear n the remains of ie a 
- Fig. 6, section of style from which the pollen has been remo Fig. 7, 
hair partly turned out of place a hair in normal position 
: 
sue; frd, fibro-vascular bundle. < 37 
tissue, i 
Brae: : : - “yng : ilen- , 
_ extine; 1, intine; ¢, stratified local t ickening of intine. Fig. 11, a po ig. 1 
showing vegetative nucleus, m; p, a beginning pollen t X 366. : “~~ 
part of the contents of a crushed pollen-spore; gn, generative nucleus; ™, 
ve nu- 
etative nucleus. < 13, a cultivated poll ; hese le of 
cleu 50 if 14 & pollen ‘tube penetrating the stigma; P,P; fs 16, 
ig. 15 i 
a pollen tube penetrating to the embryo-sac: pollen tube; s, synerg 
organized ; kn, kn (male and female proneclel ?), the germ nucleus afte 
first division. < 356, 
4 
7 
being mounted in pee the sections become — 
