360 BOTANICAL GAZETTE. 
+o 0 Slag 
tively long time. Cultures were made of Asplenium and Onoelea 
Struthiopteris in 1884, from spores sown August 31. They grew 
rapidly and soon developed quantities of antheridia, but in the 
ease of Asplenium, no archegonia. As cold weather came on, 
although the prothallia were kept in the house, growth ceased al- 
most entirely, but was resumed in the spring, the prothallia ap- 
pearing perfectly healthy. Large numbers then developed arche- 
gonia, and subsequently young plants, but some of them are still 
oy 14) perfectly vigorous, and growing. 
e 
bryo apparently ever develops perfectly. : 
Tn the older prothallia of Onoclea Struthiopteris, especially 
early in the spring, large quantities of starch were observed. 
EXPLANATION or Phare IX.—Figs. 1-5, successive stages in germinates 
of the spore of Onoclea sensibilis, < 150. Fig. 6, an older prothallium of 
same, showing the apical cell , and marginal papille (p). < 80. Fig.% 
yery small male prothallia of Asplenium tf --feemina antheridia (a). X 150. 
Fi » large male prothallia of the sa ig. 10, a male pro- 
8-9, 1 me. X 150. P 
thallium, about three months old, of Onoclea Struthiopteris. < 60. Fig. 11, m® 
ture prothallium of Cystopteris fragilis. < 5. 
On the Appearance of. the Relation of Ovary and Perianth i 
the Development of Dicotyledons.' 
BY JOHN M. COULTER. 
Two years ago I read bef . . n the 
; ore this section a paper apr 
“Development of the Dandelion,” an organogenic study, in which 
: incidentally appeared that the first character to show itself was 
at of the inferior Ovary. Since then this hint has been some 
io 
i 
Read before the American Association for the Advancement of Science, August, 18%- 
