386 BOTANICAL GAZETTE [JUNE 
of spindle threads dragged out by the knife; anilin-safranin gentian-violet, 
iron-haematoxylin. L. +5 
Fic. 35. Spindle sectioned near the cath part of one pole remaining ; 
anilin-safranin gentian-violet. L. +g Z. 
6. Daughter star stage ns nace radiations around the 
poles ; eiilin: safranin-gentian-violet. L. 7g Z. 1 
36a. Another section of the same 
F1G. 37. Chromosome from reduction nucleus showing twisted condition 
and late transverse division of chromatin granules; loose mother skein ; 
anilin-safranin gentian-violét. L. ys Z. 12 
Fic. 38. Mature typical chromosome ; chromatin granules have dis- 
appeared ; anilin-safranin gentian-violet. L. jy Z. 12. 
Fics. 39-42. Chromosomes at beginning of metakinesis, showing method 
of untwisting ; anilin-safranin gentian-violet. L. +g Z. 12 
Fic. 43-47. Chromosomes in metakinesis stage showing method of 
untwisting and indicating transverse division ; anilin-safranin gentian violet, 
iron- Sipsoggt age Lis py Zs 18 
G. 48. Single chromosome showing apparent transverse division and 
nig character of the twisted chromosome; anilin-safranin gentian-violet. 
FIGs. bes Half chromosomes after division in early daughter star; the 
pole end is in igor case at the top; anilin-safranin gentian-violet, iron-haema- 
toxylin. L. yy Z. 18. Compare figs. 57 and ¢ 
Fic. 58. Bee star in megaspore ; oe cuaiianpbeves at the poles 
from which extend a series of polar radiations ; anilin-safranin gentian-violet. 
L. +e Z. 12. 
Fic. 59. Daughter skein showing further development of radiations from 
the pole; dark bodies are probably micronucleoli ; anilin-safranin gentian- 
violet. L.+4 L.8 
Fic. 60. Daughter skein woe final arrangement of polar radiations ; 
avilin-safranin gentian-violet. L. +4 
1G. 61. Close daughter skein ‘onus disappearance of radiations and 
beginning of fusion of chromosomes to form the network ; anilin-safranin 
gentian-violet. L. 34 L. 8. 
Fic. 62. Upper nucleus in a two-celled embryo-sac; beginning of divi- 
sion; remains of central spindle still prominent; anilin-safranin gentian- 
violet, iron-haematoxylin. L. +y Z 
Fic. 63. Upper nucleus of two-celled sac, showing radiations probably 
Seas from two poles; anilin-safranin gentian-violet, iron- -haematoxylin. 
L. yy 2. 6: 
Fic. 64. Lower nucleus of the same sac ; the sections are diagon 
Fic. 65. Upper nucleus of two-celled sac; incept of spindle and are 
$ Mons spindle ; anilin-safranin gentian-violet, iron-haematoxylin. L. Ts 

