128 BOTANICAL GAZETTE; [ June, 
new. Liebig regarded these bodies as possessing the nature 
of ferments,” but Hartig first indicated the probable breaking 
up of reserve albumin into crystallenic compounds.” — Pfefler 
in his thorough discussion of the appearance of asparagin, 
already referred to, ascribed its origin to the original albu- 
min present, and pointed out that in the change C and 
were set free, either to be exhaled or adapted to the building 
of new tissue.™ 
The latter supposition seemed the more probable, since 
the asparagin which collected in etiolated growths disap- 
peared again when they were exposed to light, and album 
increased proportionally. 
Schulze’s discovery of so many other bodies homole 
to asparagin, served to strengthen and broaden the applcé 
tion of the theory of the transformation of albumin. H© 
showed farther that in the germs where the secondary P!0- 
ducts accumulated, there was an increase of H,SO, corres 
ponding to the amount of S set free from the broken dow? 
albumin molecules,® and Pfeffer regards the exhaled CO2 
as also coming from this source.” 2 
_ The dependence of these bodies upon the absence of 
nitrogen-free substances for their formation seems also clearly 
indicated by the observations upon etiolated growths, 2? 
Oo BN ae 
p. 363. 
78), p- 
