194 BOTANICAL GAZETTE. [ August, 
in Zygnema, these are colored dark blue. Methyl-violet, dahlia and 
mauvein color the protoplasm and nucleus, and_are specially valuable im 
the study of the latter. In some cases they are also precipitated in the 
cell-sip. Chrysoidin appears to color only the protoplasm. The follow- 
ing are some of the objects that were used : root-hairs of Trianea Bogaten- 
sis, Cucurbita, Tradescantia zebrina; stamen-hairs of various species of 
Tradescantia; Spirogyra spp., Zygnema spp.; roots of Lemna minor; 
leaves of Elodea (Anacharis) Canadensis, Vallisneria spiralis; pollen- 
tubes of Hemerocallis spp., Tradescantia Virginica, Scilla spp.; sperma- 
tozoids of Chara. , 
The objects are placed in a solution varying from .002 % to .001 %, 
varying with the nature of the cell-wall and the time of immersion. Root- 
hairs are usually especially delicate, and the solution should be very 
dilute or the immersion very brief. 
most cases objects were selected where there was marked proto; = 
plasmic streaming, as this is the best means of determining whether the 
cell is alive ornot. Itis surprising how deeply the protoplasm or nucleus 
may be stained without materially affecting the streaming. For a demon- 
stration of the staining of the protoplasm the root-hairs of Trianea were 
found to be specially favorable on account of their large size and the rapi 
streaming, as well as the readiness with which the color is absorbed.— 
UGLAS H. CampPBeEtt, Tiibingen. 
H. W. Ravenel.—Henry William Ravenel died at Aiken, 8. C., July 
Ith. This is indeed sad news to all American botanists, for among ther 
John’s, Berkley, S. ©., May 19, 1814. After receiving the usual high 
school training he entered the South Carolina College, and graduated 
John’s for twenty years. In 1835 he was married to Miss Elizabeth Gil- 
liard Snowden, of St. John’s. His wife died in 1855, leaving a family of 
“ix children, four of whom still survive. In 1858 he married Miss Mary 
Huger Dawson, of Charleston, who, with five children, all daughters, *Ut 
_ qyves to mourn their irreparable loss. In 1853 he removed to Aiken, 8: 
