1887. | BOTANICAL GAZETTE, 241 
nary dissecting microscope, it was easy to inoculate new cultures. The 
gelatine was of the ordinary composition in daily use in the laboratory, 
viz: ten per cent. gelatine, ten per cent. grape sugar, Liebig’s “ Fleisch 
Extract” added to give a yellowish brown color, and neutralized with so- 
dium carbonate. Such a mixture is solid at 25° ©. ' 
For further culture the isolated gelatine plate colonies were inocu- 
lated into sterilized solutions consisting of an extract made by boiling 
200 grams of yeast in a liter of water, filtering, and adding ten per cent. 
of grape sugar. In sucha solution an inoculation of a few yeast cells 
usually increased in from+twenty-four to forty-eight hours sufliciently to 
cover i 
white sediment. The cultures were most strong and active at the end of 
forty-eight hours. The supernatant fluid was then poured off, leaving 
the yeast deposit comparatively dry, twenty cc. of sterilized water added, 
and in this condition transfer to the sugar solution undergoing observa- 
extract of yeast as a nutritive solution, pure cultures were repeatedly ob- 
tained which excited as active fermentation as the fresh yeast from the 
breweries, a result not always obtained by the use of artificial nutritive 
solutions. The original gelatine plate cultures, on account of their rapid 
growth, were useless after thirty-six hours, and to avoid a constant re- 
newal of the process, as well as the introduction of different species of 
saccharomycetes, inoculations were made into gelatine tubes. The cult- 
ures thus obtained produced characteristic, elegant, ivory-white colonies 
of 3-6 mm. in diameter, and then further development ceased. In this 
state they retained their vitality, and were constantly referred to as a 
“ource of inoculating material for two months. Probably they remained 
“1gorous much longer, as saccharomycetes are well known to do, but at 
this time my need of them came to an end. a 
uch a dormant vegetative state might be favorable to the production 
of spores, which, according to the prescribed methods, I have had diffi- 
culty in obtaining. At least, for the object desired, the method given 
was found very convenient and successful_—W. E. STONE, Gottingen. 
The preparation of agaries for the herbarium.—It will be generally 
admitted that the wretched condition of most specimens of fleshy fungi in 
herbaria and published exsiccatz makes them practically worthless for 
Purposes of comparison and identification. The purpose of this note is 
‘0 call attention to a practicable process for greatly improving the quality 
of such specimens and so rendering them really valuable. ey 
In 1880, G. Herpell, of St Goar, Germany, { ei intl of his 
method for the preparation of herbarium specimens of fleshy fungi, and 
from 1881 to 1884 issued illustrations of his method in the form of four 
Small fascicles of Agaricini, under the title, “Sammlung priparirter_Hut- 
—__“Keicles of Agaricini, under the title, “Sammlung priiparirter Hut 
1 
Das Pripariren und Einlegen der Hutpilze, Bonn, 1880. 
tion was easy, by means of a pipette. By this method, and the use of the 
