136 O’NEAL: EMBRYO SAC DEVELOPMENT IN OENOTHERA 
and Professor Mottier has had the progeny under observation for 
ninegenerations. During thistime the plants have been constant 
for the characters pointed out by De Vries. During the last two 
seasons, summers of 1920 and 1921, the writer has made a 
careful examination of the leaf forms in search of the deserens 
type, but no individuals have been found with unusually broad 
leaves. There is considerable variation in the time of flowering, 
Fic. 1. Young seedlings of Oenothera rubrinervis. 
as well as a slight color difference in the flowers, but certainly 
no more than is to be expected in the range of a single species. 
Our blossoms show the crinkled edges described by Lutz (20) 
for the Amsterdam plant. This character varies considerably 
with the individual plants and seems to be influenced by various 
climatic conditions. 
e plants used in this study were grown from seeds sown 
in a box in the greenhouse in January. When the seedlings 
