﻿^^9*^-] 
  BOTANICAL 
  GAZETTE. 
  II 
  

  

  ever 
  in 
  getting 
  numerous 
  Irish 
  moss 
  cultures 
  of 
  the 
  fungus, 
  

   and 
  I 
  have 
  also 
  grown 
  it 
  in 
  water 
  both 
  ordinary 
  and 
  steril- 
  

   ized, 
  and 
  in 
  hanging 
  drops 
  as 
  well 
  as 
  in 
  drops 
  merely 
  placed 
  

   upon 
  a 
  slide. 
  

  

  In 
  my 
  next 
  experiments 
  leaves, 
  freshly 
  picked 
  from 
  the 
  

   plant, 
  were 
  used 
  and 
  kept 
  fresh 
  in 
  a 
  beaker 
  of 
  water, 
  which 
  

   was 
  placed 
  under 
  a 
  bell 
  glass, 
  where 
  there 
  was 
  a 
  second 
  

   beaker 
  of 
  steaming 
  water, 
  which 
  was 
  occasionally 
  renewed 
  

   so 
  that 
  the 
  atmosphere 
  was 
  kept 
  warm 
  and 
  moist," 
  thus 
  mak- 
  

   ing 
  the 
  conditions 
  highly 
  favorable. 
  Bacteriological 
  experi- 
  

   ments 
  under 
  these 
  conditions 
  failed 
  as 
  in 
  all 
  previous 
  experi- 
  

   ments. 
  On 
  the 
  other 
  hand, 
  experiments 
  with 
  the 
  fungus 
  

   were 
  entirely 
  successful. 
  If 
  a 
  drop 
  of 
  Irish 
  moss, 
  placed 
  on 
  

   the 
  surface 
  of 
  a 
  leaf 
  is 
  inoculated 
  with 
  spores 
  of 
  the 
  fungus 
  

   mentioned 
  above, 
  a 
  disease 
  spot 
  will 
  appear 
  in 
  about 
  two 
  

   days, 
  unless 
  in 
  some 
  way 
  the 
  drop 
  has 
  dried 
  up, 
  or 
  been 
  

   otherwise 
  disturbed. 
  If 
  the 
  drop 
  has 
  simply 
  dried 
  up, 
  it 
  is 
  

   only 
  necessary 
  to 
  moisten 
  it 
  again, 
  and 
  the 
  spot 
  will 
  soon 
  

   appear. 
  About 
  the 
  third 
  or 
  fourth 
  day 
  the 
  gonidiophores 
  

   appear, 
  and 
  soon 
  bear 
  compact 
  bunches 
  of 
  gonidia, 
  the 
  whole 
  

   forming 
  a 
  downy 
  growth 
  on 
  both 
  sides 
  of 
  the 
  leaf. 
  It 
  makes 
  

   no 
  difference 
  whether 
  the 
  original 
  spores 
  are 
  sown 
  in 
  a 
  

   drop 
  on 
  the 
  upper 
  or 
  the 
  under 
  side 
  of 
  the 
  leaf. 
  In 
  either 
  

   case 
  the 
  disease 
  is 
  produced. 
  I 
  have 
  substituted 
  drops 
  of 
  

   water 
  for 
  the 
  Irish 
  moss 
  as 
  a 
  culture 
  medium 
  with 
  equal 
  suc- 
  

   cess, 
  and 
  to 
  exclude 
  contamination, 
  drops 
  of 
  sterilized 
  water 
  

   were 
  used 
  with 
  which 
  I 
  also 
  obtained 
  excellent 
  results. 
  

  

  To 
  prove 
  beyond 
  all 
  doubt 
  that 
  the 
  fungus 
  is 
  the 
  cause 
  of 
  

   the 
  disease, 
  the 
  following 
  experiment 
  was 
  made 
  : 
  Into 
  a 
  tube 
  

   of 
  sterilized 
  nutrient 
  gelatine 
  some 
  fungus 
  spores 
  were 
  intro- 
  

   duced 
  on 
  the 
  tip 
  of 
  a 
  sterilized 
  needle. 
  The 
  contents 
  of 
  the 
  

   tube 
  were 
  then 
  " 
  plated 
  " 
  according 
  to 
  Koch's 
  method. 
  The 
  

   fungus 
  grows 
  luxuriantly 
  in 
  this 
  medium, 
  and 
  can 
  thus 
  be 
  

   obtained 
  free 
  from 
  bacteria 
  or 
  other 
  fungi. 
  Spores 
  are 
  less 
  

   quickly 
  produced 
  on 
  the 
  rich 
  gelatine 
  than 
  on 
  the 
  compara- 
  

   tively 
  poor 
  Irish 
  moss. 
  Inoculations 
  made 
  with 
  spores 
  from 
  

   a 
  pure 
  culture 
  of 
  this 
  kind 
  readily 
  produced 
  the 
  characteristic 
  

   spots, 
  while 
  blank 
  control 
  experiments 
  showed 
  nothing 
  what- 
  

  

  ever. 
  

  

  The 
  fungus 
  appeared 
  to 
  me 
  to 
  be 
  identical 
  with 
  the 
  Bo- 
  

  

  _ 
  trytis 
  

  

  M 
  

  

  ing 
  upon 
  the 
  Lilium 
  candidum 
  in 
  England, 
  and 
  from 
  a 
  speci- 
  

  

  Wai 
  

  

  m 
  % 
  

  

  lA 
  Lily 
  Disease, 
  by 
  H. 
  Marshall 
  Ward, 
  M. 
  A,, 
  F, 
  R. 
  S.. 
  F. 
  L. 
  S. 
  Annals 
  of 
  Botany, 
  vol. 
  

   \o, 
  7, 
  Nov.. 
  1888. 
  

  

  